Department of Bioresource Engineering, McGill University, Ste-Anne-de-Bellevue, QC, H9X 3V9, Canada.
Deptartment of Chemical Engineering, Université Laval, Québec, QC, G1V 0A6, Canada.
Appl Microbiol Biotechnol. 2022 Jun;106(11):4029-4039. doi: 10.1007/s00253-022-11984-1. Epub 2022 May 24.
The use of waste stream residues as feedstock for material production simultaneously helps reduce dependence on fossil-based resources and to shift toward a circular economy. This study explores the conversion of food waste into valuable chemicals, namely, bio-pigments. Here, a simulated food waste feedstock was converted into pigments via solid-state fermentation with the filamentous fungus Talaromyces albobiverticillius (NRRL 2120). Pigments including monascorubrin, rubropunctatin, and 7-(2-hydroxyethyl)-monascorubramine were identified as products of the fermentation via ultra-performance liquid chromatography coupled with quadrupole-time-of-flight electrospray ionization mass spectrometry. Pigments were obtained at concentrations of 32.5, 20.9, and 22.4 AU/gram dry substrate for pigments absorbing at 400, 475, and 500 nm, respectively. Pigment production was further enhanced by co-culturing T. albobiverticillius with Trichoderma reesei (NRRL 3652), and ultimately yielded 63.8, 35.6, and 43.6 AU/gds at the same respective wavelengths. This represents the highest reported production of pigments via solid-state fermentation of a non-supplemented waste stream feedstock. KEY POINTS: • Simulated food waste underwent solid-state fermentation via filamentous fungi. • Bio-pigments were obtained from fermentation of the simulated food waste. • Co-culturing multiple fungal species substantially improved pigment production.
将废物流残渣用作生产原料，可同时帮助减少对化石基资源的依赖，并向循环经济转型。本研究探索了将食物垃圾转化为有价值的化学品，即生物色素。在这里，通过丝状真菌白地霉（NRRL 2120）的固态发酵，将模拟食物垃圾原料转化为色素。通过超高效液相色谱-四极杆飞行时间电喷雾电离质谱联用，鉴定出包括红色素、rubropunctatin 和 7-(2-羟乙基）-monascorubramine 在内的色素作为发酵产物。色素在吸收波长为 400nm、475nm 和 500nm 的条件下，浓度分别达到 32.5、20.9 和 22.4AU/克干基质。通过白地霉与里氏木霉（NRRL 3652）共培养，进一步提高了色素产量，在相同的相应波长下，色素产量分别达到 63.8、35.6 和 43.6AU/gds。这代表了通过非补充废物流原料的固态发酵生产色素的最高报道产量。关键点： • 模拟食物垃圾通过丝状真菌进行固态发酵。 • 从发酵模拟食物垃圾中获得生物色素。 • 共培养多种真菌物种可显著提高色素产量。
