Žitek Taja, Postružnik Vesna, Knez Željko, Golle Andrej, Dariš Barbara, Knez Marevci Maša
Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engi-neering, University of Maribor, Maribor, Slovenia.
Faculty of Medicine, University of Maribor, Maribor, Slovenia.
Front Bioeng Biotechnol. 2022 May 10;10:897185. doi: 10.3389/fbioe.2022.897185. eCollection 2022.
L. flower heads are known for their antioxidant, antimicrobial, and anticancer activity. The aim of this work was to optimize the process of supercritical CO extraction, to achieve high extraction yield and high content of biologically active components, and to confirm the antimicrobial and anticancer activity of the extract. The influence of pressure and temperature on the total phenolic content, antioxidant activity, and proanthocyanidin content was evaluated. The pressure and temperature were found to be interdependent. A temperature of 60°C and a pressure of 30 MPa resulted in a high extraction yield, antioxidant activity and phenolic content. The content of proanthocyanidins was highest at a pressure between 18 and 24 MPa. The extracts inhibited three different microorganisms successfully; and , at concentrations ranging from 0.1 to 5.16 mg/ml and showed anticancer activity decrease up to 85% at a concentration of 0.5 mg/ml.
L. 花头以其抗氧化、抗菌和抗癌活性而闻名。这项工作的目的是优化超临界CO萃取工艺，以实现高萃取率和高生物活性成分含量，并确认提取物的抗菌和抗癌活性。评估了压力和温度对总酚含量、抗氧化活性和原花青素含量的影响。发现压力和温度相互依存。60°C的温度和30 MPa的压力导致了高萃取率、抗氧化活性和酚含量。原花青素含量在18至24 MPa的压力下最高。提取物成功抑制了三种不同的微生物；并且，在浓度范围为0.1至5.16 mg/ml时，在0.5 mg/ml的浓度下显示出高达85%的抗癌活性降低。
