在小鼠海马体中进行 AMPAR 介导电突触可塑性的光学分析。

Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus.

机构信息

Department of Pharmacology and the Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN 37232, USA.

出版信息

STAR Protoc. 2022 Jun 4;3(2):101443. doi: 10.1016/j.xpro.2022.101443. eCollection 2022 Jun 17.

DOI:10.1016/j.xpro.2022.101443

PMID:35677613

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9168154/

Abstract

Immunolabeling of surface AMPA receptors (AMPARs) can be used for or examination of synaptic scaling, a type of homeostatic plasticity. Here, we present a protocol to analyze changes in synaptic weights using immunohistochemistry for surface AMPARs coupled with optical imaging analysis. We detail immunostaining of AMPARs in mouse brain sections, followed by confocal imaging of surface AMPARs in dendritic region of hippocampal CA1. We then describe using Fiji/ImageJ and rank order plots for analyzing synaptic weight. For complete details on the use and execution of this protocol, please refer to Suzuki et al. (2021).

摘要

免疫标记表面 AMPA 受体 (AMPAR) 可用于或检查突触缩放，这是一种同型稳态可塑性。在这里，我们提出了一种使用免疫组织化学分析表面 AMPAR 结合光学成像分析来分析突触重量变化的方案。我们详细介绍了在小鼠脑切片中 AMPAR 的免疫染色，然后在海马 CA1 的树突区域进行表面 AMPAR 的共聚焦成像。然后，我们描述了使用 Fiji/ImageJ 和等级排序图来分析突触权重。有关此方案的使用和执行的完整详细信息，请参阅 Suzuki 等人。(2021 年)。