Department of Pharmacy, University of Salerno, Fisciano, SA, Italy.
ImmunePharma srl, Fisciano, SA, Italy.
Biosci Rep. 2020 Nov 27;40(11). doi: 10.1042/BSR20203284.
Lactoferrin (LAT), a multifunctional protein involved in numerous physiological functions, and the medicinal plant Pelargonium sidoides DC (PEL) have been described for their anti-inflammatory properties. Because the main advantage of natural products consists in administering them in combination rather than as single compound, we aimed to understand whether the combination of PEL and LAT, herein PELIRGOSTIM, could still prove beneficial or additive/synergistic activities during inflammatory conditions. To pursue this goal, we used macrophagic cells (J774.1) and treated them with PEL and LAT in a concentration-dependent manner. We found that PELIRGOSTIM was able to reduce the levels of reactive oxygen species (ROS) and nitrite, effects that were correlated to the release of lower levels of IL-1β after LPS treatment. In addition, the combination of PEL and LAT showed bacteriostatic activities against Staphylococcus aureus and Escherichia coli which had limited growth starting from 5 hours up to 20 hours. This effect was stronger than that observed for penicillin/streptomycin. Our results provide PELIRGOSTIM as an innovative combination of natural products capable to prevent inflammation-, oxidative stress- and microbial-related disorders.
乳铁蛋白（LAT）是一种多功能蛋白质，参与许多生理功能，药用植物天竺葵（PEL）因其抗炎特性而被描述。由于天然产物的主要优势在于联合使用而不是单一化合物，因此，我们旨在了解 PEL 和 LAT 的组合（即 PELIRGOSTIM）是否仍能在炎症条件下证明具有有益或附加/协同作用。为了实现这一目标，我们使用巨噬细胞（J774.1）并以浓度依赖的方式用 PEL 和 LAT 处理它们。我们发现 PELIRGOSTIM 能够降低活性氧（ROS）和亚硝酸盐的水平，这些作用与 LPS 处理后 IL-1β 释放水平降低有关。此外，PEL 和 LAT 的组合对金黄色葡萄球菌和大肠杆菌表现出抑菌活性，从 5 小时到 20 小时，这些细菌的生长受到限制。这种效果强于青霉素/链霉素的效果。我们的结果提供了 PELIRGOSTIM 作为一种天然产物的创新组合，能够预防炎症、氧化应激和微生物相关疾病。
