Deslex S, Negrel R, Vannier C, Etienne J, Ailhaud G
Int J Obes. 1987;11(1):19-27.
Stromal-vascular cells from the inguinal fat tissue of human (age range 1.5 month-27 years), were able to undergo adipose conversion when cultured in a medium containing insulin, transferrin and triiodothyronine. Between 10 and 20 per cent of the cells changed their morphology and accumulated lipid droplets within 10 to 15 days. In most cultures, differentiated cells were present in clusters. These clustered cells were shown by indirect immunofluorescence to contain lipoprotein lipase (located in the Golgi region) and by histochemistry to contain glycerol-3-phosphate dehydrogenase. The occurrence of both enzymes was assessed directly by determining enzyme activities and the synthesis of triacylglycerol was demonstrated by incorporation of [U-14C]glucose into lipids. Foreskin fibroblasts did not display any of these phenotypes. The development of a serum-free, chemically defined medium for the differentiation of diploid adipocyte precursors from human should be of interest for the characterization of factors involved in the stimulation or inhibition of the differentiation process.
来自人类腹股沟脂肪组织（年龄范围为1.5个月至27岁）的基质血管细胞，在含有胰岛素、转铁蛋白和三碘甲状腺原氨酸的培养基中培养时能够进行脂肪转化。10%至20%的细胞在10至15天内改变形态并积累脂滴。在大多数培养物中，分化细胞呈簇状存在。间接免疫荧光显示这些簇状细胞含有脂蛋白脂肪酶（位于高尔基体区域），组织化学显示含有甘油-3-磷酸脱氢酶。通过测定酶活性直接评估这两种酶的存在情况，并通过将[U-14C]葡萄糖掺入脂质中来证明三酰甘油的合成。包皮成纤维细胞未表现出任何这些表型。开发一种无血清、化学成分明确的培养基用于人二倍体脂肪细胞前体的分化，对于鉴定参与刺激或抑制分化过程的因子应具有重要意义。
