Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran.
Sci Rep. 2022 Jun 21;12(1):10491. doi: 10.1038/s41598-022-14501-6.
This work reports a facile and green approach to preparing AgIO nanoparticles decorated with chitosan (chitosan-AgIO). The bionanocomposite was fully characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) images, energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction analysis (XRD). The antibacterial effect of chitosan-AgIO bionanocomposite was investigated for Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus saprophyticus, Escherichia coli, and Staphylococcus aureus as pathogen microorganisms via the plate count method, disk diffusion method, and optical density (OD) measurements. The antibacterial performance of the bionanocomposite was compared with two commercial drugs (penicillin and silver sulfadiazine) and in some cases, the synthesized bionanocomposite has a better effect in the eradication of bacteria. The bionanocomposite represented great antibacterial properties. Flow cytometry was performed to investigate the mechanism of bionanocomposite as an antibacterial agent. Reactive oxygen species (ROS) production was responsible for the bactericidal mechanisms. These results demonstrate that the chitosan-AgIO bionanocomposite, as a kind of antibacterial material, got potential for application in a broad range of biomedical applications and water purification. The design and synthesis of green and biodegradable antibacterial materials with simple processes and by using readily available materials cause the final product to be economically affordable and could be scaled in different industries.
这项工作报道了一种简便、绿色的方法来制备负载壳聚糖的 AgIO 纳米粒子（壳聚糖-AgIO）。通过傅里叶变换红外（FTIR）、扫描电子显微镜（SEM）图像、能谱（EDX）和 X 射线衍射分析（XRD）对生物纳米复合材料进行了全面表征。通过平板计数法、圆盘扩散法和光密度（OD）测量，研究了壳聚糖-AgIO 生物纳米复合材料对铜绿假单胞菌、肺炎克雷伯菌、腐生葡萄球菌、大肠杆菌和金黄色葡萄球菌等病原体微生物的抗菌作用。将生物纳米复合材料的抗菌性能与两种商业药物（青霉素和磺胺嘧啶银）进行了比较，在某些情况下，合成的生物纳米复合材料在消除细菌方面效果更好。该生物纳米复合材料表现出很强的抗菌性能。通过流式细胞术研究了生物纳米复合材料作为一种抗菌剂的作用机制。活性氧（ROS）的产生是其杀菌机制的原因。这些结果表明，壳聚糖-AgIO 生物纳米复合材料作为一种抗菌材料，具有广泛的生物医学应用和水净化应用的潜力。设计和合成具有简单工艺、使用易得材料的绿色可生物降解抗菌材料，使最终产品具有经济实惠性，并可在不同行业中规模化生产。
