Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Department of Diagnostic and Interventional Radiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Biomolecules. 2022 Jun 7;12(6):796. doi: 10.3390/biom12060796.
The transcriptome of pulmonary hypertension (PH) is complex and highly genetically heterogeneous, with noncoding RNA transcripts playing crucial roles. The majority of RNAs in the noncoding transcriptome are long noncoding RNAs (lncRNAs) with less circular RNAs (circRNAs), which are two characteristics gaining increasing attention in the forefront of RNA research field. These noncoding transcripts (especially lncRNAs and circRNAs) exert important regulatory functions in PH and emerge as potential disease biomarkers and therapeutic targets. Recent technological advancements have established great momentum for discovery and functional characterization of ncRNAs, which include broad transcriptome sequencing such as bulk RNA-sequence, single-cell and spatial transcriptomics, and RNA-protein/RNA interactions. In this review, we summarize the current research on the classification, biogenesis, and the biological functions and molecular mechanisms of these noncoding RNAs (ncRNAs) involved in the pulmonary vascular remodeling in PH. Furthermore, we highlight the utility and challenges of using these ncRNAs as biomarkers and therapeutics in PH.
肺动脉高压（PH）的转录组十分复杂且具有高度的遗传异质性，非编码 RNA 转录本发挥着关键作用。非编码转录组中的大多数 RNA 为长非编码 RNA（lncRNA），较少为环状 RNA（circRNA），这是 RNA 研究领域前沿中越来越受到关注的两个特征。这些非编码转录本（尤其是 lncRNA 和 circRNA）在 PH 中发挥重要的调控作用，并成为潜在的疾病生物标志物和治疗靶点。最近的技术进步为 ncRNA 的发现和功能特征研究提供了巨大动力，其中包括广泛的转录组测序，如批量 RNA-seq、单细胞和空间转录组学，以及 RNA-蛋白/RNA 相互作用。在这篇综述中，我们总结了目前关于这些非编码 RNA（ncRNA）在 PH 肺血管重构中的分类、生物发生以及生物学功能和分子机制的研究。此外，我们还强调了将这些 ncRNA 用作 PH 生物标志物和治疗方法的实用性和挑战。
