Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
B CUBE Center for Molecular Bioengineering, TU Dresden, Dresden, Germany.
PLoS One. 2022 Jul 6;17(7):e0268297. doi: 10.1371/journal.pone.0268297. eCollection 2022.
Beyond the more conventional single-cell segmentation and tracking, single-cell cycle dynamics is gaining a growing interest in the field of cell biology. Thanks to sophisticated systems, such as the fluorescent ubiquitination-based cell cycle indicator (FUCCI), it is now possible to study cell proliferation, migration, changes in nuclear morphology and single cell cycle dynamics, quantitatively and in real time. In this work, we introduce FUCCItrack, an all-in-one, semi-automated software to segment, track and visualize FUCCI modified cell lines. A user-friendly complete graphical user interface is presented to record and quantitatively analyze both collective cell proliferation as well as single cell information, including migration and changes in nuclear or cell morphology as a function of cell cycle. To enable full control over the analysis, FUCCItrack also contains features for identification of errors and manual corrections.
除了更传统的单细胞分割和跟踪外，单细胞周期动力学在细胞生物学领域越来越受到关注。由于先进的系统，如荧光泛素化细胞周期指示剂 (FUCCI)，现在可以定量和实时研究细胞增殖、迁移、核形态变化和单细胞周期动力学。在这项工作中，我们引入了 FUCCItrack，这是一个一体化的、半自动的软件，用于分割、跟踪和可视化 FUCCI 修饰的细胞系。我们提供了一个用户友好的完整图形用户界面，用于记录和定量分析细胞的集体增殖以及单细胞信息，包括作为细胞周期函数的迁移和核或细胞形态的变化。为了实现对分析的完全控制，FUCCItrack 还包含用于识别错误和手动更正的功能。
