Team of Plant Biotechnology, Biology Department, Abdelmalek Essaadi University, Tetouan 93000, Morocco.
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy.
Molecules. 2022 Jun 21;27(13):3979. doi: 10.3390/molecules27133979.
This study aimed to investigate the phenolic profile and selected biological activities of the leaf and aerial extracts of three species, namely , , and , collected from three different places in the north of Morocco. The phenolic composition of all extracts was determined by LC coupled with photodiode array and mass spectrometry detection. Among the investigated extracts, that of aerial parts was the richest one, with a total amount of polyphenols of 9528.93 mg/kg. Up to 59 phenolic compounds were detected: 52 were positively identified and 49 quantified-11 in , 14 in , and 24 in . In terms of chemical classes, nine were phenolic acids and 43 were flavonoids, and among them, the majority belonged to the class of flavonols. The antioxidant activity of all extracts was investigated by three different in vitro methods, namely DPPH, reducing power, and Fe chelating assays; aerial part extract was the most active, with an IC of 0.142 ± 0.014 mg/mL (DPPH test) and 1.898 ± 0.056 ASE/mL (reducing power assay). Further, all extracts were non-toxic against , thus indicating their potential safety. The findings attained in this work for such Moroccan species, never investigated so far, bring novelty to the field and show them to be valuable sources of phenolic compounds with interesting primary antioxidant properties.
本研究旨在调查三种植物（ 、 和 ）叶和地上部分提取物的酚类成分和部分生物活性，这些植物均采自摩洛哥北部的三个不同地方。所有提取物的酚类成分均采用 LC 与光电二极管阵列和质谱检测联用的方法进行了测定。在所研究的提取物中，地上部分提取物的酚类含量最为丰富，总多酚含量为 9528.93mg/kg。共检测到 59 种酚类化合物：52 种得到了阳性鉴定，49 种进行了定量分析-其中 11 种存在于 中，14 种存在于 中，24 种存在于 中。从化学类别来看，有 9 种为酚酸，43 种为类黄酮，其中大多数属于黄酮醇类。采用三种不同的体外方法（DPPH、还原力和铁螯合试验）研究了所有提取物的抗氧化活性；地上部分提取物最为活跃，其 IC 为 0.142±0.014mg/mL（DPPH 试验）和 1.898±0.056ASE/mL（还原力试验）。此外，所有提取物对 均无毒性，表明其具有潜在的安全性。本研究首次对摩洛哥的这几种植物进行了调查，结果为该领域带来了新的认识，表明它们是具有有趣的初级抗氧化特性的酚类化合物的有价值来源。
