Yang Lei, Shi Jun, Wang Xiaowan, Zhang Rong
Department of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
Department of Emergency, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
Evid Based Complement Alternat Med. 2022 Jul 16;2022:2990843. doi: 10.1155/2022/2990843. eCollection 2022.
Oxidative stress and impaired autophagy are the hallmarks of cardiac aging. However, there are no specific drugs available to prevent cardiac aging. Curcumin is a natural polyphenolic drug with antioxidant, antiaging, and autophagy-promoting effects. Here, we describe the preventive role of Curcumin in cardiac aging through the induction of autophagy and the restoration of autophagy via the SIRT1/AMPK/mTOR pathway. The number of cells positive for senescence-associated -galactosidase, P53, P16, and intracellular ROS increased significantly in senescent cardiomyocytes, stimulated using D-galactose. Curcumin reversed this effect in a dose-dependent manner. Curcumin-induced autophagy increased the expression of SIRT1and phosphorylated AMPK and decreased phosphorylated mTOR in a dose-dependent manner. SIRT1-siRNA-mediated knockdown inhibited the antioxidation, antiaging, the promotion of autophagy, and the SIRT1/AMPK/mTOR pathway activation effect of curcumin. Therefore, curcumin could be an effective anticardiac aging drug.
氧化应激和自噬受损是心脏衰老的标志。然而，目前尚无预防心脏衰老的特效药物。姜黄素是一种天然多酚类药物，具有抗氧化、抗衰老和促进自噬的作用。在此，我们阐述了姜黄素通过诱导自噬以及经由SIRT1/AMPK/mTOR途径恢复自噬来预防心脏衰老的作用。使用D-半乳糖刺激衰老心肌细胞后，衰老相关β-半乳糖苷酶、P53、P16及细胞内活性氧阳性细胞数量显著增加。姜黄素呈剂量依赖性地逆转了这种效应。姜黄素诱导的自噬呈剂量依赖性地增加了SIRT1的表达及磷酸化AMPK水平，并降低了磷酸化mTOR水平。SIRT1-siRNA介导的敲低抑制了姜黄素的抗氧化、抗衰老、促进自噬及SIRT1/AMPK/mTOR途径激活作用。因此，姜黄素可能是一种有效的抗心脏衰老药物。
