Huang Ke, Wang Huihui, Xu Jia, Xu Ruiming, Liu Zelin, Li Yi, Xu Zhaoqing
Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730030, China.
Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University, Lanzhou 730030, China.
Biology (Basel). 2022 Jul 26;11(8):1115. doi: 10.3390/biology11081115.
(1) Background: The functions of the tropomyosin (TPM) family in tumors and cancers have been explored; however, striking differences have been observed. This study aims to further our understanding of the effects of TPMs in glioma, and find novel biomarkers for glioma. (2) Methods: RNA-seq data were downloaded from TCGA and GTEx. Survival analyses, Cox regression, nomogram, calibration curves, ROC curves, gene function enrichment analyses, and immune cell infiltration analyses were carried out using R. CCK8 assay, while Brdu assay, colony formation assay, and Transwell assay were used to verify the functions of TPM3 in glioma. (3) Results: TPM1/3/4 were significantly more highly expressed in glioma than that in normal tissues, while higher expression of TPM2/3/4 was correlated with a worse overall survival than lower expression of TPM2/3/4. Furthermore, bioinformatic analyses indicated that TPM3/4 could be promoting factors for poorer survival in glioma, but only TPM3 could serve as an independent prognostic factor. Gene function analyses showed that TPMs may be involved in immune responses. Moreover, further experimental investigations verified that TPM3 overexpression enhanced the proliferation and tumorigenicity of glioma. (4) Conclusions: High expression of TPM3/4 was positively correlated with poorer prognosis in glioma, and TPM3 could serve as a novel independent prognostic factor of glioma.
(1) 背景：已对原肌球蛋白（TPM）家族在肿瘤中的功能进行了探索；然而，观察到了显著差异。本研究旨在进一步了解TPM在胶质瘤中的作用，并寻找胶质瘤的新型生物标志物。(2) 方法：从TCGA和GTEx下载RNA测序数据。使用R进行生存分析、Cox回归、列线图、校准曲线、ROC曲线、基因功能富集分析和免疫细胞浸润分析。采用CCK8法，同时使用Brdu法、集落形成法和Transwell法验证TPM3在胶质瘤中的功能。(3) 结果：TPM1/3/4在胶质瘤中的表达明显高于正常组织，而TPM2/3/4高表达与总体生存率较差相关，低于TPM2/3/4表达。此外，生物信息学分析表明，TPM3/4可能是胶质瘤患者生存较差的促进因素，但只有TPM3可作为独立的预后因素。基因功能分析表明，TPM可能参与免疫反应。此外，进一步的实验研究证实，TPM3过表达增强了胶质瘤的增殖和致瘤性。(4) 结论：TPM3/4高表达与胶质瘤预后较差呈正相关，TPM3可作为胶质瘤新的独立预后因素。
