Kim Hyeon Bin, Na Eui Young, Yun Sook Jung, Lee Jee-Bum
Department of Dermatology, Chonnam National University Medical School, Gwangju, Korea.
Ann Dermatol. 2022 Aug;34(4):261-269. doi: 10.5021/ad.21.223.
Rosacea is a chronic inflammatory skin disease with a pathophysiological mechanism that remains unclear. Recently, dysregulation of the sensory nerve system has been implicated in the development of this condition.
This study aimed to investigate the effect of capsaicin on neuroinflammatory mediators in rosacea. In addition, this study aimed to evaluate the attenuating effects of capsazepine, a transient receptor potential vanilloid type 1 (TRPV1) antagonist.
We obtained skin tissue from both rosacea patients and normal individuals for an study. In addition, normal human epidermal keratinocytes (NHEKs) were cultured, and treated with capsaicin and capsazepine for an study. Quantitative changes in neuroinflammatory mediators were evaluated by semi-quantitative reverse transcription-polymerase chain reaction (PCR), real-time PCR, enzyme-linked immunosorbent assay, and immunofluorescence staining.
The data showed the increase of TRPV1, TRPV4, cathelicidin (LL37) and tumor necrosis factor-α (TNF-α) in skin tissue by real-time PCR. In addition, the data showed that cathelicidin (LL37), kallikrein-5 (KLK-5), TNF-α, vascular endothelial growth factor (VEGF), interleukin (IL)-1α, IL-1β, IL-8, and protease-activated receptor 2 (PAR2) increased in capsaicin-treated NHEKs. Capsazepine attenuated the expression of TRPV1 and other mediators, except for IL-8, in capsaicin-treated NHEKs.
We confirmed that TRPV1, TRPV4, cathelicidin (LL37) and TNF-α are increased in rosacea skin, and that capsaicin is associated with increase of neuroinflammatory mediators such as LL37, KLK-5, TNF-α, VEGF, IL-1α, IL-1β, IL-8, and PAR2. Modulators or inhibitors of neuroinflammatory mediators including TRPV1 could be potential therapeutic option in the treatment of patients with rosacea.
酒渣鼻是一种慢性炎症性皮肤病，其病理生理机制尚不清楚。最近，感觉神经系统失调被认为与这种疾病的发生有关。
本研究旨在探讨辣椒素对酒渣鼻神经炎症介质的影响。此外，本研究旨在评估瞬时受体电位香草酸受体1（TRPV1）拮抗剂辣椒平的减轻作用。
我们获取了酒渣鼻患者和正常个体的皮肤组织进行研究。此外，培养正常人表皮角质形成细胞（NHEK），并用辣椒素和辣椒平进行处理以进行研究。通过半定量逆转录-聚合酶链反应（PCR）、实时PCR、酶联免疫吸附测定和免疫荧光染色评估神经炎症介质的定量变化。
实时PCR数据显示皮肤组织中TRPV1、TRPV4、抗菌肽（LL37）和肿瘤坏死因子-α（TNF-α）增加。此外，数据显示在辣椒素处理的NHEK中，抗菌肽（LL37）、激肽释放酶-5（KLK-5）、TNF-α、血管内皮生长因子（VEGF）、白细胞介素（IL）-1α、IL-1β、IL-8和蛋白酶激活受体2（PAR2）增加。辣椒平减弱了辣椒素处理的NHEK中TRPV1和其他介质（IL-8除外）的表达。
我们证实酒渣鼻皮肤中TRPV1、TRPV4、抗菌肽（LL37）和TNF-α增加，并且辣椒素与LL37、KLK-5、TNF-α、VEGF、IL-1α、IL-1β、IL-8和PAR2等神经炎症介质的增加有关。包括TRPV1在内的神经炎症介质的调节剂或抑制剂可能是治疗酒渣鼻患者的潜在治疗选择。
