Wade C R, Jackson P G, Highton J, van Rij A M
Clin Chim Acta. 1987 May 15;164(3):245-50. doi: 10.1016/0009-8981(87)90298-1.
The concentration of lipid peroxides in the plasma and synovial fluid of 65 arthritic patients was determined using a new ion-pairing reverse phase HPLC technique. Patients with rheumatoid arthritis receiving only non-steroidal anti-inflammatory drugs, had a significantly higher mean concentration of lipid peroxides in synovial fluid samples (162 +/- 22.0 micrograms/l) than osteoarthritic patients (40.0 +/- 8.0 micrograms/l, p less than 0.0001). Mean concentrations in both groups correlated strongly with the level of beta-glucuronidase activity as a measure of lysosomal enzyme release (r = 0.71, p less than 0.0001). Contrary to previous reports by investigators using less specific methods, we were unable to demonstrate any increase in plasma levels of lipid peroxides in the rheumatoid patient. Treatment of rheumatoid arthritis with D-penicillamine was associated with a significant reduction of lipid peroxide levels (83.2 +/- 11.5 micrograms/ml, p less than 0.002), suggesting that this drug may function as an oxygen radical scavenger in the joint cavity. These results give further support to the concept of oxygen-free radicals playing an important role in the pathogenesis of chronic inflammatory disorders.
采用一种新的离子对反相高效液相色谱技术，测定了65例关节炎患者血浆和滑液中脂质过氧化物的浓度。仅接受非甾体抗炎药治疗的类风湿关节炎患者，其滑液样本中脂质过氧化物的平均浓度（162±22.0微克/升）显著高于骨关节炎患者（40.0±8.0微克/升，p＜0.0001）。两组的平均浓度与作为溶酶体酶释放指标的β-葡萄糖醛酸酶活性水平密切相关（r = 0.71，p＜0.0001）。与以往使用特异性较差方法的研究者报告相反，我们未能证明类风湿患者血浆中脂质过氧化物水平有任何升高。用D-青霉胺治疗类风湿关节炎与脂质过氧化物水平显著降低相关（83.2±11.5微克/毫升，p＜0.002），这表明该药物可能在关节腔中起到氧自由基清除剂的作用。这些结果进一步支持了氧自由基在慢性炎症性疾病发病机制中起重要作用这一概念。
