将赖特疏螺旋体轴丝进行对流免疫电泳作为梅毒的诊断试验。
Counterimmunoelectrophoresis of Reiter Treponeme Axial filaments as a diagnostic test for syphilis.
作者信息
Nell E E, Hardy P H
出版信息
J Clin Microbiol. 1978 Aug;8(2):148-52. doi: 10.1128/jcm.8.2.148-152.1978.
Abstract
Purified axial filaments from the cultivable Reiter treponeme, previously shown to share an antigenic component with pathogenic Treponemia pallidum, were evaluated as antigen in a diagnostic test for syphilis. Antibody to the filaments was revealed by counterimmunoelectrophoresis. Conditions that produced optimal results in the test were established. A total of 343 sera from normal individuals, biological false-positive reactors, and from patients in the different stages of syphilis were subjected to the test. The results indicate the test to be sensitive and highly specific for detecting treponemal antibodies in human syphilis.
摘要
从可培养的赖特密螺旋体中提纯的轴丝，先前已证明其与致病性梅毒螺旋体有共同的抗原成分，在梅毒诊断试验中作为抗原进行了评估。通过对流免疫电泳检测针对轴丝的抗体。确定了在该试验中产生最佳结果的条件。对来自正常个体、生物学假阳性反应者以及处于梅毒不同阶段患者的总共343份血清进行了该试验。结果表明该试验在检测人类梅毒中的密螺旋体抗体方面具有敏感性和高度特异性。
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Counterimmunoelectrophoresis of Reiter Treponeme Axial filaments as a diagnostic test for syphilis.将赖特疏螺旋体轴丝进行对流免疫电泳作为梅毒的诊断试验。
J Clin Microbiol. 1978 Aug;8(2):148-52. doi: 10.1128/jcm.8.2.148-152.1978.
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本文引用的文献
1
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Br J Vener Dis. 1962 Sep;38(3):131-3. doi: 10.1136/sti.38.3.131.
2
[Description of a technic simultaneously combining electrophoresis and immunological precipitation in gel: electrosyneresis].[一种在凝胶中同时结合电泳和免疫沉淀的技术描述：电凝缩]
Biochim Biophys Acta. 1959 Jul;34:258-60. doi: 10.1016/0006-3002(59)90261-6.
3
[New methods of serodiagnosis of syphilis. III. Use of a protein antigen of Treponema pallidum (Reiter strain) in the complement fixation test].梅毒血清学诊断的新方法。III. 梅毒螺旋体（赖特菌株）蛋白抗原在补体结合试验中的应用
Ned Tijdschr Geneeskd. 1957 Aug 31;101(35):1615-7.
4
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Am J Syph Gonorrhea Vener Dis. 1953 Mar;37(2):137-50.
5
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
Immunochemistry. 1971 Sep;8(9):871-4. doi: 10.1016/0019-2791(71)90454-x.
6
Evaluation of the counter-immunoelectrophoresis technique in syphilis serology.梅毒血清学中对流免疫电泳技术的评估。
Br J Vener Dis. 1974 Apr;50(2):101-3. doi: 10.1136/sti.50.2.101.
7
Demonstration of precipitins against a treponemal antigen by counter-immunoelectrophoresis.通过对流免疫电泳检测针对梅毒螺旋体抗原的沉淀素
Lancet. 1972 May 6;1(7758):996-7. doi: 10.1016/s0140-6736(72)91161-0.
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