不同酒精浓度下物种产生乙醛的能力。
Capacity of species to produce acetaldehyde at various concentrations of alcohol.
作者信息
Reddy Mamatha G S, Kakodkar Pradnya, Nayanar Gayatri
机构信息
Department of Oral Pathology and Microbiology, D.Y. Patil Vidyapeeth's, Dr. D.Y. Patil Dental College and Hospital, Pune, Maharashtra, India.
Department of Research, D.Y. Patil Vidyapeeth, Pune, Maharashtra, India.
出版信息
J Oral Maxillofac Pathol. 2022 Apr-Jun;26(2):161-165. doi: 10.4103/jomfp.jomfp_494_20. Epub 2022 Jun 28.
BACKGROUND
Along with tobacco use, alcohol consumption is one of the crucial factors for oral cancer. Acetaldehyde (ACH), a byproduct of alcohol, is reported as carcinogenic. One of the producers of ACH from alcohol is Candida species. The aim of the study was to quantify the ACH produced by species at various concentrations of alcohol.
MATERIALS AND METHODS
Clinical isolates of , namely , and and ATCC 18,804, were subjected to various concentrations of alcohol. Alcohol dehydrogenase and ACH were estimated using spectrophotometry and headspace gas chromatography, respectively.
RESULTS
Out of all three clinical isolates, produced more ACH (412.1 μM) at 10 mM alcohol concentration by 10colony-forming unit/ml followed by (233 μM) and (53.7 μM). of clinical isolate and ATCC species (222 μM) did not show much difference.
CONCLUSION
The study results conclude that species are capable of producing carcinogenic levels of ACH on exposure to various concentrations of alcohol.
背景
与烟草使用一样，饮酒是口腔癌的关键因素之一。乙醛（ACH）是酒精的一种副产物，据报道具有致癌性。酒精产生ACH的生产者之一是念珠菌属。本研究的目的是量化念珠菌属在不同酒精浓度下产生的ACH。
材料与方法
将白色念珠菌、光滑念珠菌、热带念珠菌的临床分离株以及白色念珠菌ATCC 18804暴露于不同浓度的酒精中。分别使用分光光度法和顶空气相色谱法测定酒精脱氢酶和ACH。
结果
在所有三种临床分离株中，白色念珠菌在酒精浓度为10 mM时，每毫升菌落形成单位为10时产生的ACH更多（412.1 μM），其次是光滑念珠菌（233 μM）和热带念珠菌（53.7 μM）。临床分离株白色念珠菌和ATCC菌株（222 μM）之间没有太大差异。
结论
研究结果表明，念珠菌属在接触不同浓度酒精时能够产生致癌水平的ACH。
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