Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano & Soft Materials & Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC), Soochow University, Suzhou, 215123, China.
Nat Commun. 2022 Sep 1;13(1):5127. doi: 10.1038/s41467-022-32837-5.
Bacteria can bypass the blood-brain barrier (BBB), suggesting the possibility of employment of bacteria for combating central nervous system diseases. Herein, we develop a bacteria-based drug delivery system for glioblastoma (GBM) photothermal immunotherapy. The system, which we name as 'Trojan bacteria', consists of bacteria loaded with glucose polymer and photosensitive ICG silicon-nanoparticles. In an orthotopic GBM mouse model, we demonstrate that the intravenously injected bacteria bypass the BBB, targeting and penetrating GBM tissues. Upon 808 nm-laser irradiation, the photothermal effects produced by ICG allow the destruction of bacterial cells and the adjacent tumour cells. Furthermore, the bacterial debris as well as the tumour-associated antigens promote antitumor immune responses that prolong the survival of GBM-bearing mice. Moreover, we demonstrate the residual bacteria are effectively eliminated from the body, supporting the potential therapeutic use of this system.
细菌可以绕过血脑屏障（BBB），这表明细菌有可能被用于治疗中枢神经系统疾病。在此，我们开发了一种基于细菌的用于治疗胶质母细胞瘤（GBM）的光热免疫治疗的药物递送系统。该系统被命名为“特洛伊细菌”，由负载葡萄糖聚合物和光敏性吲哚菁绿硅纳米颗粒的细菌组成。在原位 GBM 小鼠模型中，我们证明了静脉注射的细菌可以绕过 BBB，靶向并穿透 GBM 组织。在 808nm 激光照射下，ICG 产生的光热效应可破坏细菌细胞和邻近的肿瘤细胞。此外，细菌碎片以及肿瘤相关抗原可促进抗肿瘤免疫反应，从而延长荷瘤小鼠的生存期。此外，我们还证明了体内的残留细菌可被有效清除，这支持了该系统的潜在治疗用途。
