Institute of Process and Particle Engineering, Graz University of Technology, Inffeldgasse 13, 8010, Graz, Austria.
Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010, Graz, Austria.
ChemSusChem. 2022 Nov 22;15(22):e202201468. doi: 10.1002/cssc.202201468. Epub 2022 Sep 26.
Photo- and biocatalysis show many advantages as more sustainable solutions for the production of fine chemicals. In an effort to combine the benefits and the knowledge of both these areas, a continuous photobiocatalytic setup was designed and optimized to carry out whole-cell biotransformations within cells of the cyanobacterium Synechocystis sp. PCC 6803 expressing the gene of the ene-reductase YqjM from B. subtilis. The effect of the light intensity and flow rate on the specific activity in the stereoselective reduction of 2-methyl maleimide was investigated via a design-of-experiments approach. The cell density in the setup was further increased at the optimal operating conditions without loss in specific activity, demonstrating that the higher surface area/volume ratio in the coil reactor improved the illumination efficiency of the process. Furthermore, different reactor designs were compared, proving that the presented approach was the most cost- and time-effective solution for intensifying photobiotransformations within cyanobacterial cells.
光催化和生物催化作为生产精细化学品的更可持续的解决方案显示出许多优势。为了结合这两个领域的优势和知识，设计并优化了一种连续的光生物催化装置，以在表达来自枯草芽孢杆菌的 ene-还原酶 YqjM 基因的蓝藻集胞藻 PCC 6803 细胞内进行全细胞生物转化。通过实验设计方法研究了光强度和流速对 2-甲基马来酰亚胺立体选择性还原的比活性的影响。在最佳操作条件下，进一步增加了装置中的细胞密度，而比活性没有损失，这表明在螺旋反应器中更高的表面积/体积比提高了该过程的光照效率。此外，还比较了不同的反应器设计，证明所提出的方法是在蓝藻细胞内强化光生物转化的最具成本效益和时间效益的解决方案。
