中国荆门蜱病毒的地理分布及系统发育分析
Geographical distribution and phylogenetic analysis of Jingmen tick virus in China.
作者信息
Pang Zheng, Jin Yuting, Pan Mei, Zhang Yuli, Wu Zhen, Liu Lin, Niu Guoyu
机构信息
School of Public Health, Weifang Medical University, Weifang 261053, China.
Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China.
出版信息
iScience. 2022 Aug 25;25(9):105007. doi: 10.1016/j.isci.2022.105007. eCollection 2022 Sep 16.
Jingmen tick virus (JMTV) is a novel tick-borne segmented RNA virus that is closely related to un-segmental RNA virus in evolution. It has been confirmed that JMTV could be a causative agent of human disease. In this study, a total of 3658 ticks were sampled from 7 provinces of China and then divided into 545 pools according to the location and species. QRT-PCR and nested PCR were performed to confirm the presence of JMTV. The results showed JMTV was identified in 5 out of 7 provinces with an average infection rate of 1.4% (51/3658). Phylogenetic analysis indicated that all JMTV strains identified in this study were closely related to each other and formed a well-supported sub-lineage. Our results provide molecular evidence of JMTV in different species of ticks from endemic and non-endemic regions and demonstrate that JMTV, as a natural foci pathogen, may be widely distributed all over China.
荆门蜱病毒（JMTV）是一种新型蜱传分段RNA病毒，在进化上与非分段RNA病毒密切相关。已证实JMTV可能是人类疾病的病原体。在本研究中，从中国7个省份采集了共计3658只蜱，然后根据地点和种类分为545组。进行了实时定量PCR（QRT-PCR）和巢式PCR以确认JMTV的存在。结果显示，在7个省份中的5个省份发现了JMTV，平均感染率为1.4%（51/3658）。系统发育分析表明，本研究中鉴定出的所有JMTV毒株彼此密切相关，并形成了一个有充分支持的亚谱系。我们的结果提供了JMTV在流行和非流行地区不同种类蜱中的分子证据，并表明JMTV作为一种自然疫源性病原体，可能在中国广泛分布。
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