Department of Prosthodontics, Peking University School and Hospital of Stomatology, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; National Center of Stomatology, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; National Clinical Research Center for Oral Diseases, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China.
Department of Prosthodontics, Peking University School and Hospital of Stomatology, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; National Center of Stomatology, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; National Clinical Research Center for Oral Diseases, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China; Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun Avenue South, Haidian District, Beijing 100081, PR China.
Carbohydr Polym. 2022 Dec 1;297:120027. doi: 10.1016/j.carbpol.2022.120027. Epub 2022 Aug 27.
Delayed inflammatory reaction and poor osteogenesis are the two main causes of failure for bone-defect healing. Accordingly, in the present study, a dual-responsive hydrogel composite was successfully fabricated in which near-infrared (NIR)-light-responsive polydopamine-coated magnesium-calcium carbonate microspheres are incorporated into a thermo-responsive hydroxybutyl chitosan hydrogel to provide sequential delivery of the anti-inflammatory drug aspirin and osteogenic bone morphogenetic protein 2 (BMP-2). By initially releasing aspirin rapidly, the hydrogel composite efficiently ameliorates early-stage inflammatory reaction and promotes transition to the regenerative phase. Then, the hydrogel composite allows NIR-light-responsive release of BMP-2, which maximizes its osteoinductive effects. Using an SD rat calvaria-defect model, the sequential and controllable release achieved by the hydrogel is demonstrated to promote new-bone formation. Thus, the current study provides an efficient alternative strategy for developing multifunctional therapeutic biomaterials for bone tissue engineering.
延迟的炎症反应和较差的成骨作用是骨缺损愈合失败的两个主要原因。因此，在本研究中，成功制备了一种双重响应水凝胶复合材料，其中将近红外（NIR）光响应聚多巴胺涂覆的镁钙碳酸盐微球掺入到温敏性羟丁基壳聚糖水凝胶中，以顺序递呈抗炎药物阿司匹林和成骨骨形态发生蛋白 2（BMP-2）。通过最初快速释放阿司匹林，水凝胶复合材料可有效改善早期炎症反应并促进向再生阶段的转变。然后，水凝胶复合材料允许 NIR 光响应释放 BMP-2，从而最大程度地发挥其成骨诱导作用。使用 SD 大鼠颅骨缺损模型，证明水凝胶的顺序和可控释放可促进新骨形成。因此，本研究为开发用于骨组织工程的多功能治疗性生物材料提供了一种有效的替代策略。
