Mhamadi Moufid, Badji Aminata, Dieng Idrissa, Gaye Alioune, Ndiaye El Hadji, Ndiaye Mignane, Mhamadi Moundhir, Touré Cheikh Talibouya, Mbaye Mouhamed Rassoul, Barry Mamadou Aliou, Ndiaye Oumar, Faye Babacar, Ba Fatimata Amadou, Diop Boly, Ndiaye Mamadou, Fall Mathioro, Sagne Samba Niang, Fall Gamou, Loucoubar Cheikh, Fausther-Bovendo Hugues, Sall Amadou Alpha, Kobinger Gary, Faye Ousmane, Diallo Mawlouth, Faye Oumar
Institut Pasteur de Dakar, Virology Department, Dakar 12900, Senegal.
Parasitology Department, Université Cheikh Anta Diop de Dakar, Dakar 10700, Senegal.
Trop Med Infect Dis. 2022 Oct 21;7(10):324. doi: 10.3390/tropicalmed7100324.
Crimean-Congo hemorrhagic fever virus (CCHFV) is widespread in Asia, Europe, and Africa. In Senegal, sporadic cases of CCHFV have been reported since 1960. Bordering Mauritania in northeastern Senegal, Agnam is an arid area in the region of Matam where CCHFV is endemic, which harbors a pastoralist community. Given the drought conditions of Agnam, inhabitants are in constant movement with their animals in search of pasture, which brings them into contact with pathogens such as arboviruses. To identify CCHFV in this area, we established a One Health site in order to analyze animal livestock, ticks and human samples collected over a one-year period by qRT-PCR and ELISA. Our analysis showed one (1/364) patient carried anti-CCHFV IgM and thirty-seven carried anti-CCHFV IgG (37/364). In livestock, anti-CCHFV IgG was detected in 13 (38.24%) of 34 sentinel sheep. The risk of CCHFV infection increased significatively with age in humans (-value = 0.00117) and sheep (-value = 1.18 × 10). Additional risk factors for CCHFV infection in sheep were dry seasons (-value = 0.004) and time of exposure (-value = 0.007). Furthermore, we detected a total of three samples with CCHFV RNA within and tick species. Our results highlighted the usefulness of a One Health survey of CCHFV in pastoral communities at risk of arboviruses.
克里米亚-刚果出血热病毒（CCHFV）在亚洲、欧洲和非洲广泛传播。自1960年以来，塞内加尔已报告了CCHFV的散发病例。阿格南位于塞内加尔东北部，与毛里塔尼亚接壤，是马塔姆地区的一个干旱地区，CCHFV在该地区流行，这里居住着一个游牧社区。鉴于阿格南的干旱状况，居民们带着他们的牲畜不断迁徙以寻找牧场，这使他们接触到诸如虫媒病毒等病原体。为了在该地区识别CCHFV，我们建立了一个“同一健康”监测点，以便通过定量逆转录聚合酶链反应（qRT-PCR）和酶联免疫吸附测定（ELISA）分析在一年时间内收集的动物家畜、蜱虫和人类样本。我们的分析显示，有一名患者（1/364）携带抗CCHFV IgM，37人携带抗CCHFV IgG（37/364）。在家畜中，在34只哨兵羊中有13只（38.24%）检测到抗CCHFV IgG。在人类（P值=0.00117）和绵羊（P值=1.18×10）中，CCHFV感染风险随年龄显著增加。绵羊感染CCHFV的其他风险因素是旱季（P值=0.004）和接触时间（P值=0.007）。此外，我们在 种和 种蜱虫中总共检测到3份含有CCHFV RNA的样本。我们的结果突出了对有虫媒病毒感染风险的游牧社区进行CCHFV“同一健康”调查的有用性。
