NIA-Layton Alzheimer's Disease Research Center, Oregon Health & Science University, Portland, Oregon, USA.
Department of Pathology, Oregon Health & Science University, Portland, Oregon, USA.
J Neuropathol Exp Neurol. 2022 Dec 19;82(1):57-70. doi: 10.1093/jnen/nlac103.
Postmortem (PM) magnetic resonance imaging (MRI) can serve as a bridge between in vivo imaging and histology by connecting MRI observed macrostructural findings to histological staining and microstructural changes. Data were acquired from 20 formalin-fixed brains including T2, T1, PD, and T2*-weighted images of left hemispheres and 6-mm-thick coronal slices. Tissue slices were bisected, aligned to MR images and used to guide histological sampling. Markers of myelin and oligodendroglia alterations were semiquantitatively rated and compared within white matter hyperintensities (WMHs) and normal-appearing white matter. Tissue priors were created from 3T in vivo data and used to guide segmentation of WMH. PM WMH and hemisphere volumes were compared to volumes derived from in vivo data. PM T2 WMH and T1 hemisphere volumes were correlated with in vivo 3T FLAIR WMH and T1 hemisphere volumes. WMH showed significant myelin loss, decreased GFAP expression and increased vimentin expression. MR-visible perivascular spaces and cortical microvascular lesions were successfully captured on histopathological sections. PM MRI can quantify cerebrovascular disease burden and guide tissue sampling, allowing for more comprehensive characterization of cerebrovascular disease that may be used to study etiologies of age-related cognitive change.
死后（PM）磁共振成像（MRI）可以通过将 MRI 观察到的宏观结构发现与组织学染色和微观结构变化联系起来，在体内成像和组织学之间架起桥梁。该研究从 20 个福尔马林固定的大脑中获取数据，包括左半球的 T2、T1、PD 和 T2*-加权图像，以及 6 毫米厚的冠状切片。组织切片被对半切开，与 MRI 图像对齐，并用于指导组织学取样。在脑白质高信号（WMH）和正常表现的白质内，对髓鞘和少突胶质细胞改变的标志物进行半定量评分和比较。组织先验从 3T 体内数据中创建，并用于指导 WMH 的分割。将 PM WMH 和脑半球体积与体内数据得出的体积进行比较。PM T2 WMH 和 T1 脑半球体积与体内 3T FLAIR WMH 和 T1 脑半球体积相关。WMH 显示出明显的髓鞘丢失、GFAP 表达减少和波形蛋白表达增加。MR 可见的血管周围间隙和皮质微血管病变在组织病理学切片上成功捕获。PM MRI 可以定量评估脑血管疾病负担并指导组织取样，从而更全面地描述脑血管疾病，可用于研究与年龄相关的认知变化的病因。
