高度催化的金属有机框架负载银纳米团簇与特定适配体相结合用于痕量多巴胺的表面增强拉曼光谱定量分析。

Highly catalysis MOF supported Ag nanoclusters coupled with specific aptamer for SERS quantitative assay of trace dopamine.

作者信息

Shi Jinling, Li Jingjing, Liang Aihui, Jiang Zhiliang

机构信息

Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, 541004, China.

出版信息

Talanta. 2022 Aug 1;245:123468. doi: 10.1016/j.talanta.2022.123468. Epub 2022 Apr 6.

DOI:10.1016/j.talanta.2022.123468

PMID:35405447

Abstract

A cerium metal organic framework-loaded silver nanocluster (MOF) is synthesized by a facile stirring procedure with trimesic acid, cerium nitrate, silver nitrate and NaBH, which exhibites strong catalytic activity in the indicated reaction of HAuCl-sodium lactate (SL). MOF can sensitively detect dopamine (DA) by surface-enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) techniques with a detection concentration range of 0.01-0.25 nmol/L and a detection limit of 0.008 nmol/L. Based on the specific binding of aptamer (Apt)-DA and the catalytic amplification strategy of MOF on HAuCl-SL, a sensitive and convenient DA platform for dual-mode detection of SERS and RRS is constructed. In addition, the platform is successfully applied to detect DA in human serum with satisfactory recoveries (95.7-102%).

摘要

通过均苯三甲酸、硝酸铈、硝酸银和硼氢化钠的简单搅拌程序合成了一种铈金属有机框架负载银纳米团簇（MOF），其在氯金酸 - 乳酸钠（SL）的指定反应中表现出很强的催化活性。MOF可以通过表面增强拉曼散射（SERS）和共振瑞利散射（RRS）技术灵敏地检测多巴胺（DA），检测浓度范围为0.01 - 0.25 nmol/L，检测限为0.008 nmol/L。基于适体（Apt） - DA的特异性结合以及MOF对氯金酸 - SL的催化放大策略，构建了一种用于SERS和RRS双模检测的灵敏便捷的DA检测平台。此外，该平台成功应用于检测人血清中的DA，回收率令人满意（95.7 - 102%）。