Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowka St. 166, 02-786 Warsaw, Poland.
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland.
Toxins (Basel). 2022 Oct 29;14(11):743. doi: 10.3390/toxins14110743.
The presence of deoxynivalenol (DON) in feed may increase intestinal barrier permeability. Disturbance of the intestinal barrier integrity may affect the absorption of antibiotics used in animals. Since the bioavailability of orally administered antibiotics significantly affects their efficacy and safety, it was decided to evaluate how DON influences the absorption of the most commonly used antibiotics in pigs, i.e., amoxicillin (AMX) and doxycycline (DOX). The studies were conducted using jejunal explants from adult pigs. Explants were incubated in Ussing chambers, in which a buffer containing DON (30 µg/mL), AMX (50 µg/mL), DOX (30 µg/mL), a combination of AMX + DON, or a combination of DOX + DON was used. Changes in transepithelial electrical resistance (TEER), the flux of transcellular and intracellular transport markers, and the flux of antibiotics across explants were measured. DON increased the permeability of small intestine explants, expressed by a reduction in TEER and an intensification of transcellular marker transport. DON did not affect AMX transport, but it accelerated DOX transport by approximately five times. The results suggest that DON inhibits the efflux transport of DOX to the intestinal lumen, and thus significantly changes its absorption from the gastrointestinal tract.
脱氧雪腐镰刀菌烯醇（DON）存在于饲料中可能会增加肠道屏障通透性。肠道屏障完整性的破坏可能会影响动物使用的抗生素的吸收。由于口服抗生素的生物利用度会显著影响其疗效和安全性，因此决定评估 DON 如何影响猪中最常用的抗生素（即阿莫西林（AMX）和多西环素（DOX））的吸收。该研究使用成年猪的空肠切片进行。将切片在 Ussing 室中孵育，其中缓冲液中含有 DON（30 µg/mL）、AMX（50 µg/mL）、DOX（30 µg/mL）、AMX+DON 混合物或 DOX+DON 混合物。测量跨上皮电阻（TEER）的变化、细胞内和细胞间转运标志物的通量以及抗生素穿过切片的通量。DON 通过降低 TEER 和增强细胞间标志物转运来增加小肠切片的通透性。DON 不影响 AMX 的转运，但它将 DOX 的转运速度加快了约五倍。结果表明，DON 抑制了 DOX 向肠腔的外排转运，从而显著改变了 DOX 从胃肠道的吸收。
