State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, People's Republic of China.
Department of Oncology, Yueyang Central Hospital, Yueyang 414000, People's Republic of China.
Biomed Mater. 2022 Nov 23;17(6). doi: 10.1088/1748-605X/aca24c.
As a typical metal-organic framework (MOF), Mg-MOF74 can release biocompatible Mgwhen the framework is degraded, and it has the potential to be used as filler in the field of bone tissue engineering. However, Mg-MOF74 has poor stability in aqueous environment and limited antibacterial ability, which limit its further development and applications. In this work, MgCu-MOF74 particles with different Cu content were synthesized through a facile one-step hydrothermal method. The physicochemical properties and water stability of the synthesized powders were characterized. The osteogenic potential of the MgCu-MOF74 particles on human osteogenic sarcoma cells (SaOS-2) was evaluated. The hybrid MgCu-MOF74 exhibited favorable water stability. These results indicated that MgCu-MOF74 enhanced cellular viability, alkaline phosphatase levels, collagen (COL) synthesis and osteogenesis-related gene expression. Moreover, the samples doped with Cuwere more sensitive to the acidic microenvironment produced by bacteria, and exhibited stronger antibacterial ability than Mg-MOF74. In conclusion, MgCu-MOF-74 with good water stability, osteogenic ability and antibacterial ability, which could be attributed to the doping of Cu. Hence, MgCu-MOF74 shows great potential as a novel medical bio-functional fillers for the treatment of bone defects.
作为一种典型的金属有机骨架（MOF），Mg-MOF74 可以在骨架降解时释放出生物相容性的 Mg，因此具有在骨组织工程领域作为填充剂的潜力。然而，Mg-MOF74 在水相环境中稳定性差，抗菌能力有限，限制了其进一步的发展和应用。在这项工作中，通过简便的一步水热法合成了不同 Cu 含量的 MgCu-MOF74 颗粒。对合成粉末的物理化学性质和水稳定性进行了表征。评估了 MgCu-MOF74 颗粒对人骨肉瘤细胞（SaOS-2）的成骨潜力。MgCu-MOF74 具有良好的水稳定性。这些结果表明，MgCu-MOF74 增强了细胞活力、碱性磷酸酶水平、胶原（COL）合成和骨形成相关基因表达。此外，掺杂 Cu 的样品对细菌产生的酸性微环境更为敏感，表现出比 Mg-MOF74 更强的抗菌能力。总之，具有良好的水稳定性、成骨能力和抗菌能力的 MgCu-MOF-74 可以归因于 Cu 的掺杂。因此，MgCu-MOF74 作为一种新型的医用生物功能填充剂，具有治疗骨缺损的巨大潜力。
