Tao Jie, Zhu Shunyao, Liao Xueyuan, Wang Yu, Zhou Nazi, Li Zhan, Wan Haoyuan, Tang Yaping, Yang Sen, Du Ting, Yang Yang, Song Jinlin, Liu Rui
Department of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, China.
Department of Stem Cell and Regenerative Medicine, State Key Laboratory of Trauma, Burn and Combined Injury, Daping Hospital, Army Medical University, Chongqing, 400042, China.
Mater Today Bio. 2022 Nov 5;17:100487. doi: 10.1016/j.mtbio.2022.100487. eCollection 2022 Dec 15.
The integration of 3D bioprinting and stem cells is of great promise in facilitating the reconstruction of cranial defects. However, the effectiveness of the scaffolds has been hampered by the limited cell behavior and functions. Herein, a therapeutic cell-laden hydrogel for bone regeneration is therefore developed through the design of a void-forming hydrogel. This hydrogel is prepared by digital light processing (DLP)-based bioprinting of the bone marrow stem cells (BMSCs) mixed with gelatin methacrylate (GelMA)/dextran emulsion. The 3D-bioprinted hydrogel can not only promote the proliferation, migration, and spreading of the encapsulated BMSCs, but also stimulate the YAP signal pathway, thus leading to the enhanced osteogenic differentiation of BMSCs. In addition, the in vivo therapeutic assessments reveal that the void-forming hydrogel shows great potential for BMSCs delivery and can significantly promote bone regeneration. These findings suggest that the unique 3D-bioprinted void-forming hydrogels are promising candidates for applications in bone regeneration.
3D生物打印与干细胞的整合在促进颅骨缺损修复方面具有巨大潜力。然而，支架的有效性受到细胞行为和功能有限的阻碍。在此，通过设计一种形成空隙的水凝胶，开发了一种用于骨再生的负载治疗性细胞的水凝胶。这种水凝胶是通过基于数字光处理（DLP）的骨髓干细胞（BMSCs）与甲基丙烯酸明胶（GelMA）/葡聚糖乳液混合的生物打印制备的。3D生物打印的水凝胶不仅可以促进封装的BMSCs的增殖、迁移和扩散，还可以刺激YAP信号通路，从而导致BMSCs的成骨分化增强。此外，体内治疗评估表明，形成空隙的水凝胶在BMSCs递送方面具有巨大潜力，并且可以显著促进骨再生。这些发现表明，独特的3D生物打印形成空隙的水凝胶是骨再生应用的有前途的候选材料。
