Department of Surgery - Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Spectro Coating Corporation, Flock Coatings & Short Cut Fibers, Leominster, MA, 01453, USA.
Adv Mater. 2023 Feb;35(5):e2207335. doi: 10.1002/adma.202207335. Epub 2022 Dec 19.
Drawing inspiration for biomaterials from biological systems has led to many biomedical innovations. One notable bioinspired device, Velcro, consists of two substrates with interlocking ability. Generating reversibly interlocking biomaterials is an area of investigation, as such devices can allow for modular tissue engineering, reversibly interlocking biomaterial interfaces, or friction-based coupling devices. Here, a biaxially interlocking interface generated using electrostatic flocking is reported. Two electrostatically flocked substrates are mechanically and reversibly interlocked with the ability to resist shearing and compression forces. An initial high-throughput screen of polyamide flock fibers with varying diameters and fiber lengths is conducted to elucidate the roles of different fiber parameters on scaffold mechanical properties. After determining the most desirable parameters via weight scoring, polylactic acid (PLA) fibers are used to emulate the ideal scaffold for in vitro use. PLA flocked scaffolds are populated with osteoblasts and interlocked. Interlocked flocked scaffolds improved cell survivorship under mechanical compression and sustained cell viability and proliferation. Additionally, the compression and shearing resistance of cell-seeded interlocking interfaces increased with increasing extracellular matrix deposition. The introduction of extracellular matrix-reinforced interlocking interfaces may serve as binders for modular tissue engineering, act as scaffolds for engineering tissue interfaces, or enable friction-based couplers for biomedical applications.
从生物系统中汲取灵感来设计生物材料已经催生了许多医学创新。一种著名的仿生装置是维可牢尼龙搭扣，它由两个具有互锁能力的基底组成。可生成具有可逆互锁能力的生物材料是一个研究领域，因为这样的装置可以用于模块化组织工程、具有可逆互锁能力的生物材料界面或基于摩擦力的耦合装置。本文报道了一种使用静电植绒技术生成的双轴互锁界面。两个静电植绒基底通过机械和可逆的方式互锁，能够抵抗剪切和压缩力。首先进行了一个具有不同直径和纤维长度的聚酰胺植绒纤维的高通量筛选实验，以阐明不同纤维参数对支架机械性能的作用。通过权重评分确定了最理想的参数后，使用聚乳酸（PLA）纤维来模拟体外应用的理想支架。将 PLA 植绒支架与成骨细胞进行混合并互锁。互锁的植绒支架在机械压缩下提高了细胞存活率，维持了细胞的活力和增殖。此外，细胞接种的互锁界面的压缩和剪切阻力随着细胞外基质沉积的增加而增加。引入具有细胞外基质增强互锁界面的方法可以作为模块化组织工程的结合剂，用作工程组织界面的支架，或为生物医学应用提供基于摩擦力的耦合器。
