Brown N J, Rumsey R D, Read N W
Sub Department of Human Gastrointestinal Physiology and Nutrition, The University, Sheffield.
Gut. 1987 Jul;28(7):849-54. doi: 10.1136/gut.28.7.849.
Excreted hydrogen analysis was used to measure stomach to caecum transit time of the head of a test meal in 120 rats fed by gavage. Results were compared with the distribution of a labelled test meal in the gastrointestinal tract of rats killed at different time intervals after gavage. Values for stomach to caecum transit were compatible with the distribution of labelled meals in 91% of animals, although in the remainder the hydrogen concentration had not risen even though food residues were in the caecum when the animals were killed. The technique gave reproducible results; the coefficients of variation for four studies carried out in each of six animals varied between 4 and 14%. A meal consisting of homogenised baked beans had a significantly shorter stomach to caecum transit time (88.1 +/- 4.5 min; mean +/- SE; n = 21; p less than 0.001) than an equivalent volume of Complan/lactulose (180.9 +/- 8.7 min; n = 13). This technique was used to investigate the effect of ileal infusion of a fat emulsion (20% Intralipid) via a chronically implanted intestinal cannula on the stomach to caecum transit time of a bean meal, in a series of paired studies carried out in six rats. Stomach to caecum transit time was significantly delayed during ileal infusion of 20% Intralipid compared with the control infusion of an isotonic saline solution (218.3 +/- 21 min v 106.7 +/- 33 min Intralipid v saline; n = 6; p less than 0.001).
采用呼出氢气分析法测定了120只经口灌喂的大鼠一次试验餐头部从胃至盲肠的转运时间。将结果与灌喂后在不同时间间隔处死的大鼠胃肠道中标记试验餐的分布情况进行了比较。在91%的动物中，胃至盲肠的转运时间值与标记餐的分布情况相符，尽管在其余动物中，即使在处死动物时食物残渣已在盲肠中，但氢气浓度仍未升高。该技术给出了可重复的结果；在六只动物中的每只动物上进行的四项研究的变异系数在4%至14%之间。与等量体积的康宝莱/乳果糖（180.9±8.7分钟；n = 13）相比，由均质烤豆组成的一餐从胃至盲肠的转运时间显著缩短（88.1±4.5分钟；平均值±标准误；n = 21；p<0.001）。在对六只大鼠进行的一系列配对研究中，采用该技术研究了通过长期植入的肠套管回肠输注脂肪乳剂（20%英脱利匹特）对豆餐从胃至盲肠转运时间的影响。与等渗盐溶液的对照输注相比，回肠输注20%英脱利匹特期间，胃至盲肠的转运时间显著延迟（英脱利匹特组为218.3±21分钟，盐水组为106.7±33分钟；n = 6；p<0.001）。
