Wu Yang, Li Ming, Su Hao, Chen Huaying, Zhu Yonggang
School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, China.
State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou, China.
Int J Bioprint. 2022 Oct 28;9(1):628. doi: 10.18063/ijb.v9i1.628. eCollection 2023.
The major apparatuses used for three-dimensional (3D) bioprinting include extrusion-based, droplet-based, and laser-based bioprinting. Numerous studies have been proposed to fabricate bioactive 3D bone tissues using different bioprinting techniques. In addition to the development of bioinks and assessment of their printability for corresponding bioprinting processes, and success of the bioprinted constructs, such as their mechanical properties, cell viability, differentiation capability, immune responses, and osseointegration, have been explored. In this review, several major considerations, challenges, and potential strategies for bone bioprinting have been deliberated, including bioprinting apparatus, biomaterials, structure design of vascularized bone constructs, cell source, differentiation factors, mechanical properties and reinforcement, hypoxic environment, and dynamic culture. In addition, up-to-date progress in bone bioprinting is summarized in detail, which uncovers the immense potential of bioprinting in re-establishing the 3D dynamic microenvironment of the native bone. This review aims to assist the researchers to gain insights into the reconstruction of clinically relevant bone tissues with appropriate mechanical properties and precisely regulated biological behaviors.
用于三维（3D）生物打印的主要设备包括基于挤压、基于液滴和基于激光的生物打印。已经有许多研究提出使用不同的生物打印技术制造具有生物活性的3D骨组织。除了生物墨水的开发及其在相应生物打印过程中的可打印性评估外，还探索了生物打印构建体的成功之处，如它们的机械性能、细胞活力、分化能力、免疫反应和骨整合。在这篇综述中，对骨生物打印的几个主要考虑因素、挑战和潜在策略进行了探讨，包括生物打印设备、生物材料、血管化骨构建体的结构设计、细胞来源、分化因子、机械性能和增强、缺氧环境以及动态培养。此外，还详细总结了骨生物打印的最新进展，揭示了生物打印在重建天然骨的3D动态微环境方面的巨大潜力。这篇综述旨在帮助研究人员深入了解具有适当机械性能和精确调控生物行为的临床相关骨组织的重建。
