Hoene Miriam, Zhao Xinjie, Machann Jürgen, Birkenfeld Andreas L, Heni Martin, Peter Andreas, Niess Andreas, Moller Anja, Lehmann Rainer, Xu Guowang, Weigert Cora
Institute for Clinical Chemistry and Pathobiochemistry, Department for Diagnostic Laboratory Medicine, University Hospital Tübingen, 72076 Tübingen, Germany.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Metabolites. 2022 Dec 22;13(1):15. doi: 10.3390/metabo13010015.
Physical exercise is a powerful measure to prevent cardiometabolic diseases. However, the individual response to lifestyle interventions is variable and cannot, to date, be predicted. N-Lactoylphenylalanine (Lac-Phe) produced during exercise has recently been shown to mediate weight loss in obese mice. Lac-Phe could also contribute to, and potentially explain differences in, the effectiveness of exercise interventions in humans. Sedentary overweight and obese subjects completed an 8-week supervised endurance exercise intervention (n = 22). Before and after the intervention, plasma levels of Lac-Phe were determined by UHPLC-MS in the resting state and immediately after an acute bout of endurance exercise. Adipose tissue volume was quantified using MRI. Acute exercise caused a pronounced increase in Lac-Phe, both before and after the intervention. Higher levels of Lac-Phe after acute exercise were associated with a greater reduction in abdominal subcutaneous and, to a lower degree, visceral adipose tissue during the intervention. Lac-Phe produced during physical activity could contribute to weight loss by acting as a signaling molecule that regulates food intake, as previously shown in mice. Quantification of Lac-Phe during an exercise test could be employed as a tool to predict and potentially improve the individual response to exercise-based lifestyle interventions in overweight humans and those with obesity.
体育锻炼是预防心脏代谢疾病的有力措施。然而，个体对生活方式干预的反应存在差异，迄今为止尚无法预测。最近研究表明，运动过程中产生的N-乳酰苯丙氨酸（Lac-Phe）可介导肥胖小鼠体重减轻。Lac-Phe也可能导致并潜在解释运动干预对人类有效性的差异。久坐的超重和肥胖受试者完成了一项为期8周的有监督耐力运动干预（n = 22）。在干预前后，通过超高效液相色谱-质谱法（UHPLC-MS）测定静息状态下以及一次急性耐力运动后即刻的血浆Lac-Phe水平。使用磁共振成像（MRI）对脂肪组织体积进行定量分析。急性运动导致干预前后Lac-Phe显著增加。急性运动后较高水平的Lac-Phe与干预期间腹部皮下脂肪组织的更大减少相关，对内脏脂肪组织的减少程度较低。如先前在小鼠中所示，体育活动过程中产生的Lac-Phe可能通过作为调节食物摄入的信号分子来促进体重减轻。在运动测试期间对Lac-Phe进行定量分析可作为一种工具，用于预测并潜在改善超重和肥胖人群对基于运动的生活方式干预的个体反应。
