Cédric Yamssi, Christelle Nadia Noumedem Anangmo, Sylvain Raoul Simeni Njonnou, Berinyuy Samuel, Azizi Mounvera Abdel, Jemimah Sandra Tientcheu Noutong, Aboubakar Sidiki Ngouyamsa Nsapkain, Payne Vincent Khan
Department of Biomedical Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39, Bambili, Cameroon.
Department of Microbiology, Haematology and Immunology Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, Cameroon.
J Trop Med. 2023 Jan 20;2023:9504296. doi: 10.1155/2023/9504296. eCollection 2023.
There are about 13 parasitic infections that are responsible for significant morbidity and mortality but have not received the attention they deserve; thus, they are now known as "neglected tropical diseases" (NTDs). This study was aimed at evaluating the antihelminthic activities of using an automated high-throughput method.
The antihelminthic activity effect of the extracts against . was determined using an automated high-throughput method. For the egg-hatching test, 100 L of embryonated egg suspension (60 eggs) was added to 100 L of various concentrations of extracts, levamisole, and 1.5% DMSO in a 96-well round-bottom microtitre plate. After mixing, the 96-well microplate was placed in WMicroTracker and incubated for 24 h at 25°C; the movements were recorded every 30 minutes. The same procedure was used for the larval motility assays, where 100 L of L1 or L2 larvae (50 larvae) were put in contact with 100 L of various concentrations of extracts.
The ovicidal activity (hatching) had an IC of 1.4 mg/mL for the ethanol extract. The aqueous and ethanol extracts of . showed larvicidal activity on the L1 larvae with IC of 1.85 mg/mL and 2.4 mg/mL, respectively, as well as on the L2 larvae with IC values of 1.08 mg/mL and 1.02 mg/mL for the aqueous and ethanol extracts, respectively. These results showed that the aqueous extract exhibited a stronger inhibitory power on the hatching rate of parasites than ethanol extracts, while the contrary effect was observed for the larval motility assays.
This study provides scientific data on the use of . by the local population for the treatment of helminthiases. However, and toxicity tests are necessary to assess its activity and safety.
约有13种寄生虫感染导致了显著的发病率和死亡率，但未得到应有的关注；因此，它们现在被称为“被忽视的热带病”（NTDs）。本研究旨在使用自动化高通量方法评估[提取物名称未给出]的抗蠕虫活性。
使用自动化高通量方法测定提取物对[寄生虫名称未给出]的抗蠕虫活性效果。对于虫卵孵化试验，将100μL胚胎化虫卵悬液（60个虫卵）加入到96孔圆底微量滴定板中100μL不同浓度的提取物、左旋咪唑和1.5%二甲基亚砜中。混合后，将96孔微量滴定板置于WMicroTracker中，在25°C下孵育24小时；每30分钟记录一次运动情况。幼虫活力测定采用相同程序，将100μL L1或L2幼虫（50条幼虫）与100μL不同浓度的提取物接触。
乙醇提取物的杀卵活性（孵化）IC50为1.4mg/mL。[植物名称未给出]的水提取物和乙醇提取物对L1幼虫均表现出杀幼虫活性，IC50分别为1.85mg/mL和2.4mg/mL，对L2幼虫的IC50值，水提取物和乙醇提取物分别为1.08mg/mL和1.02mg/mL。这些结果表明，水提取物对寄生虫孵化率的抑制能力比乙醇提取物更强，而在幼虫活力测定中观察到相反的效果。
本研究为当地居民使用[植物名称未给出]治疗蠕虫病提供了科学数据。然而，[具体内容未明确]和毒性试验对于评估其活性和安全性是必要的。
