Antihelminthic Activity of on Using an Automated High-Throughput Method.

BACKGROUND

There are about 13 parasitic infections that are responsible for significant morbidity and mortality but have not received the attention they deserve; thus, they are now known as "neglected tropical diseases" (NTDs). This study was aimed at evaluating the antihelminthic activities of using an automated high-throughput method.

METHODS

The antihelminthic activity effect of the extracts against . was determined using an automated high-throughput method. For the egg-hatching test, 100 L of embryonated egg suspension (60 eggs) was added to 100 L of various concentrations of extracts, levamisole, and 1.5% DMSO in a 96-well round-bottom microtitre plate. After mixing, the 96-well microplate was placed in WMicroTracker and incubated for 24 h at 25°C; the movements were recorded every 30 minutes. The same procedure was used for the larval motility assays, where 100 L of L1 or L2 larvae (50 larvae) were put in contact with 100 L of various concentrations of extracts.

RESULTS

The ovicidal activity (hatching) had an IC of 1.4 mg/mL for the ethanol extract. The aqueous and ethanol extracts of . showed larvicidal activity on the L1 larvae with IC of 1.85 mg/mL and 2.4 mg/mL, respectively, as well as on the L2 larvae with IC values of 1.08 mg/mL and 1.02 mg/mL for the aqueous and ethanol extracts, respectively. These results showed that the aqueous extract exhibited a stronger inhibitory power on the hatching rate of parasites than ethanol extracts, while the contrary effect was observed for the larval motility assays.

CONCLUSION

This study provides scientific data on the use of . by the local population for the treatment of helminthiases. However, and toxicity tests are necessary to assess its activity and safety.