Zhang Huimin, Xu Zhenxia, Chen Wenchao, Huang Fenghong, Chen Shouwen, Wang Xu, Yang Chen
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Oil Crops and Lipids Process Technology National and Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, Hubei University, Wuhan, China.
Front Nutr. 2023 Jan 16;9:1081717. doi: 10.3389/fnut.2022.1081717. eCollection 2022.
Taking antibiotics would interfere with gut microbiota and increase the risk of opportunistic pathogen infection and inflammation.
In this study, 36 male C57BL/6 mice were divided into 4 groups ( = 9) to investigate whether two kinds of algal oil could alleviate the intestinal damage induced by CS (Ceftriaxone sodium). These algal oils were obtained from sp. cultures using Yeast extract (YE) and Rapeseed meal (RSM) as substrate, respectively. All tested mice were administrated with CS for 8 days and then the colon pathological morphology, the expression levels of inflammatory factors and the gut microbial profile were analyzed in mice supplemented with or without algal oil.
The results showed that both YE and RSM algal oils markedly reduced mucosal damage and intestinal inflammatory response in CS-treated mice by inhibiting the pro-inflammatory cytokine tumor necrosis factor (TNF)-α, interleukin (IL)-6 and myeloperoxidase (MPO) activity. In addition, fluorescence immunohistochemistry showed that the tight junction protein ZO-1 was increased in mice supplemented with YE and RSM algal oil. Furthermore, YE algal oil promoted the beneficial intestinal bacteria such as and S24_7 compared with the CS group, while supplementation with RSM algal oil enriched the Robinsoniella. Spearman's correlation analysis exhibited that and were positively correlated with IL-6 but negatively correlated with IL-10.
This study suggested that supplementation with algal oil could alleviate intestinal inflammation by regulating gut microbiota and had a protective effect on maintaining intestinal barrier against antibiotic-induced damage in mice.
服用抗生素会干扰肠道微生物群，增加机会性病原体感染和炎症的风险。
在本研究中，将36只雄性C57BL/6小鼠分为4组（每组n = 9），以研究两种藻油是否能减轻头孢曲松钠（CS）诱导的肠道损伤。这些藻油分别以酵母提取物（YE）和菜籽粕（RSM）为底物，从 sp. 培养物中获得。所有受试小鼠均接受CS处理8天，然后分析补充或未补充藻油的小鼠的结肠病理形态、炎症因子表达水平和肠道微生物谱。
结果表明，YE和RSM藻油均通过抑制促炎细胞因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和髓过氧化物酶（MPO）活性，显著减轻CS处理小鼠的黏膜损伤和肠道炎症反应。此外，荧光免疫组织化学显示，补充YE和RSM藻油的小鼠紧密连接蛋白ZO-1增加。此外，与CS组相比，YE藻油促进了有益肠道细菌如 和S24_7的生长，而补充RSM藻油则富集了罗氏菌属。Spearman相关性分析表明， 和 与IL-6呈正相关，但与IL-10呈负相关。
本研究表明，补充藻油可通过调节肠道微生物群减轻肠道炎症，并对维持小鼠肠道屏障免受抗生素诱导的损伤具有保护作用。
