Department of Metallurgical and Materials Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul 34220, Turkey.
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul 34722, Turkey.
Int J Mol Sci. 2020 Oct 29;21(21):8082. doi: 10.3390/ijms21218082.
Natural calcium phosphates derived from fish wastes are a promising material for biomedical application. However, their sintered ceramics are not fully characterized in terms of mechanical and biological properties. In this study, natural calcium phosphate was synthesized through a thermal calcination process from salmon fish bone wastes. The salmon-derived calcium phosphates (sCaP) were sintered at different temperatures to obtain natural calcium phosphate bioceramics and then were investigated in terms of their microstructure, mechanical properties and biocompatibility. In particular, this work is concerned with the effects of grain size on the relative density and microhardness of the sCaP bioceramics. Ca/P ratio of the sintered sCaP ranged from 1.73 to 1.52 when the sintering temperature was raised from 1000 to 1300 °C. The crystal phase of all the sCaP bioceramics obtained was biphasic and composed of hydroxyapatite (HA) and tricalcium phosphate (TCP). The density and microhardness of the sCaP bioceramics increased in the temperature interval 1000-1100 °C, while at temperatures higher than 1100 °C, these properties were not significantly altered. The highest compressive strength of 116 MPa was recorded for the samples sintered at 1100 °C. In vitro biocompatibility was also examined in the behavior of osteosarcoma (Saos-2) cells, indicating that the sCaP bioceramics had no cytotoxicity effect. Salmon-derived biphasic calcium phosphates (BCP) have the potential to contribute to the development of bone substituted materials.
从鱼废弃物中提取的天然磷酸钙是一种很有前途的生物医学应用材料。然而，其烧结陶瓷在力学和生物学性能方面尚未得到充分的表征。在这项研究中，天然磷酸钙是通过鲑鱼骨废弃物的热煅烧过程合成的。将鲑鱼来源的磷酸钙（sCaP）在不同温度下烧结，以获得天然磷酸钙生物陶瓷，然后对其微观结构、力学性能和生物相容性进行研究。特别是，这项工作关注的是晶粒尺寸对 sCaP 生物陶瓷相对密度和显微硬度的影响。当烧结温度从 1000°C 升高到 1300°C 时，烧结的 sCaP 的 Ca/P 比从 1.73 变化到 1.52。所有 sCaP 生物陶瓷的晶体相均为双相，由羟基磷灰石（HA）和磷酸三钙（TCP）组成。sCaP 生物陶瓷的密度和显微硬度在 1000-1100°C 的温度范围内增加，而在高于 1100°C 的温度下，这些性能没有明显变化。在 1100°C 烧结的样品中记录到最高的抗压强度为 116 MPa。体外生物相容性也通过骨肉瘤（Saos-2）细胞的行为进行了检查，表明 sCaP 生物陶瓷没有细胞毒性作用。源自鲑鱼的双相磷酸钙（BCP）有潜力促进骨替代材料的发展。
