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绿色化学合成的银纳米颗粒对[具体菌种1]和[具体菌种2]的抗真菌活性评估

Evaluation of Antifungal Activity of Ag Nanoparticles Synthetized by Green Chemistry against and .

作者信息

Moreno-Vargas J M, Echeverry-Cardona L M, Moreno-Montoya L E, Restrepo-Parra E

机构信息

Laboratorio de Física del Plasma, Universidad Nacional de Colombia, Sede Manizales, Manizales 170001, Colombia.

出版信息

Nanomaterials (Basel). 2023 Jan 29;13(3):548. doi: 10.3390/nano13030548.

Abstract

Silver nanoparticles (AgNPs) have aroused great interest for applications as fungicides in agriculture. This study reports the synthesis of AgNPs by green chemistry using silver nitrate (AgNO) as the precursor agent and a coriander leaf extract as the reducing agent and surfactant. The evaluation of their antifungal properties was carried out when placed in contact with and phytopathogens. The extract and AgNP characterizations were performed using UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), dynamic light scattering (DLS) and scanning electron microscopy (SEM). The evaluation of antifungal properties was carried out by exposing the phytopathogens to different concentrations of AgNPs in PDA (Potato Dextrose Agar). It was found that it was possible to identify the presence of flavones and flavonoids in the extract, compounds that were also involved in the synthesis process of AgNPs. In addition, the UV-Vis analysis of the obtained AgNPs by green chemistry showed resonance peaks at around 428 nm. Furthermore, a high distribution of AgNP sizes, with high concentrations of below 100 nm, was identified, according to DLS measurements. Using SEM images, the information provided by DLS was confirmed, and a crystallite size of 29.24 nm was determined with the help of XRD measurements. Finally, when exposing the phytopathogens to the action of AgNPs, it was concluded that, at a concentration of 1 mg/mL AgNPs, their growth was totally inhibited.

摘要

银纳米颗粒(AgNPs)作为农业杀菌剂的应用引起了极大的关注。本研究报告了采用绿色化学方法合成AgNPs,以硝酸银(AgNO₃)作为前驱体试剂,用香菜叶提取物作为还原剂和表面活性剂。将其与两种植物病原体接触时,对其抗真菌特性进行了评估。使用紫外可见光谱、X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)、动态光散射(DLS)和扫描电子显微镜(SEM)对提取物和AgNP进行了表征。通过将植物病原体暴露于不同浓度的PDA(马铃薯葡萄糖琼脂)中的AgNPs来评估抗真菌特性。发现可以在提取物中鉴定出黄酮和类黄酮的存在,这些化合物也参与了AgNPs的合成过程。此外,通过绿色化学方法获得的AgNPs的紫外可见分析显示在约428nm处有共振峰。此外,根据DLS测量,确定了AgNP尺寸的高度分布,其中低于100nm的浓度很高。使用SEM图像证实了DLS提供的信息,并借助XRD测量确定了微晶尺寸为29.24nm。最后,当将植物病原体暴露于AgNPs的作用下时,得出结论,在1mg/mL AgNPs的浓度下,它们的生长被完全抑制。

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