Mhamadi Moufid, Badji Aminata, Barry Mamadou Aliou, Ndiaye El Hadji, Gaye Alioune, Ndiaye Mignane, Mhamadi Moundhir, Touré Cheikh Talibouya, Ndiaye Oumar, Faye Babacar, Diop Boly, Ndiaye Mamadou, Fall Mathioro, Diouf Andy Mahine, Sagne Samba Niang, Loucoubar Cheikh, Fausther-Bovendo Hugues, Sall Amadou Alpha, Kobinger Gary, Faye Ousmane, Diallo Mawlouth, Faye Oumar
Virology Department, Institut Pasteur de Dakar, Dakar 12900, Senegal.
Parasitology Department, Université Cheikh Anta Diop de Dakar, Dakar 10700, Senegal.
Trop Med Infect Dis. 2023 Jan 27;8(2):87. doi: 10.3390/tropicalmed8020087.
The mosquito-borne disease caused by the Rift Valley Fever Virus (RVFV) is a viral hemorrhagic fever that affects humans and animals. In 1987, RVFV emerged in Mauritania, which caused the first RVFV outbreak in West Africa. This outbreak was shortly followed by reported cases in humans and livestock in Senegal. Animal trade practices with neighboring Mauritania suggest northern regions of Senegal are at high risk for RVF. In this study, we aim to conduct a molecular and serological survey of RVFV in humans and livestock in Agnam (northeastern Senegal) by RT-PCR (reverse transcription real-time polymerase chain reaction) and ELISA (Enzyme-Linked Immunosorbent Assay), respectively. Of the two hundred fifty-five human sera, one (0.39%) tested RVFV IgM positive, while fifty-three (20.78%) tested positive for RVFV IgG. For animal monitoring, out of 30 sheep recorded and sampled over the study period, 20 (66.67%) showed seroconversion to RVFV IgG antibodies, notably during the rainy season. The presence of antibodies increased significantly with age in both groups ( < 0.05), as the force of RVF infection (FOI), increased by 16.05% per year for humans and by 80.4% per month for livestock sheep. This study supports the usefulness of setting up a One Health survey for RVF management.
由裂谷热病毒（RVFV）引起的蚊媒疾病是一种影响人类和动物的病毒性出血热。1987年，RVFV在毛里塔尼亚出现，引发了西非首次RVFV疫情。此次疫情之后不久，塞内加尔就报告了人类和牲畜感染病例。与邻国毛里塔尼亚的动物贸易活动表明，塞内加尔北部地区面临裂谷热的高风险。在本研究中，我们旨在分别通过逆转录实时聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA），对阿格南（塞内加尔东北部）的人类和牲畜进行RVFV的分子和血清学调查。在255份人类血清中，1份（0.39%）RVFV IgM检测呈阳性，而53份（20.78%）RVFV IgG检测呈阳性。对于动物监测，在研究期间记录并采样的30只绵羊中，20只（66.67%）显示血清转化为RVFV IgG抗体，特别是在雨季。两组中抗体的存在都随年龄显著增加（<0.05），因为裂谷热感染率（FOI），人类每年增加16.05%，牲畜绵羊每月增加80.4%。本研究支持开展裂谷热管理的“同一健康”调查的有用性。
