Yin Wang, Nziengui Raby Randy Bachelard, Li Yuankai, Li Zuojun, Sun Mengqing, Huang Zhi
Institute of Biomedical Engineering, School of Basic Medical Sciences, Central South University, Changsha 410017, China.
Department of Pharmacy, the Third Xiangya Hospital, Central South University, Changsha 410013, China.
Bioengineering (Basel). 2023 Jan 18;10(2):129. doi: 10.3390/bioengineering10020129.
Herein, we designed chitosan-coated FeO nanocomposites for the control release of drugs by an alternating magnetic field (AMF). The chitosan-coated FeO nanoparticles (FeO@CS) were prepared by a alkaline co-precipitation method, and then, the model drug toluidine blue (TB) was covalently grafted onto the surface of the nanocomposite by a two-step amide reaction with the thermosensitive molecule 4,4'-azobis (4-cyanovaleric acid) (ACVA) as the linker group. The prepared nanocomposites were superparamagnetic and showed high magnetization saturation (about 54.0 emu g). In vitro hydrothermal release studies showed that most parts of the TB would be effectively enclosed within the nanocarriers at lower ambient temperatures (23 or 37 °C) due to the molecular bonding of ACVA. The results of kinetic fitting of hydrothermal release data showed that TB released from nanoparticles followed first-order kinetics (R > 0.99) and the Korsemeyer-Peppas model (R > 0.99, n < 0.5). Most importantly, a single magnetron release experiment demonstrated an approximately linear relationship between the cumulative release of the drug and the duration of action of AMF (R = 0.9712). Moreover, the increase in the cumulative release of the drug can be controlled by controlling the switch of the AMF generation device. Therefore, the ACVA-modified FeO@CS nanocarrier designed in this study is a promising model for drug delivery that enables the control of drug release dose by AMF.
在此，我们设计了壳聚糖包覆的FeO纳米复合材料，用于通过交变磁场（AMF）控制药物释放。采用碱性共沉淀法制备了壳聚糖包覆的FeO纳米颗粒（FeO@CS），然后，以热敏分子4,4'-偶氮双（4-氰基戊酸）（ACVA）为连接基团，通过两步酰胺反应将模型药物甲苯胺蓝（TB）共价接枝到纳米复合材料表面。所制备的纳米复合材料具有超顺磁性，表现出高饱和磁化强度（约54.0 emu g）。体外水热释放研究表明，在较低环境温度（23或37°C）下，由于ACVA的分子键合作用，大部分TB会有效地包裹在纳米载体中。水热释放数据的动力学拟合结果表明，纳米颗粒释放的TB遵循一级动力学（R>0.99）和Korsemeyer-Peppas模型（R>0.99，n<0.5）。最重要的是，单磁控管释放实验表明药物的累积释放与AMF的作用持续时间之间存在近似线性关系（R = 0.9712）。此外，通过控制AMF发生装置的开关，可以控制药物累积释放量的增加。因此，本研究设计的ACVA修饰的FeO@CS纳米载体是一种很有前景的药物递送模型，能够通过AMF控制药物释放剂量。
