Yang Yanshen, Jia Yuanbo, Yang Qingzhen, Xu Feng
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, P.R. China.
Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, P.R. China.
Int J Bioprint. 2022 Oct 29;9(1):632. doi: 10.18063/ijb.v9i1.632. eCollection 2023.
144Three-dimensional (3D) bioprinting has become a promising approach to constructing functional biomimetic tissues for tissue engineering and regenerative medicine. In 3D bioprinting, bio-inks are essential for the construction of cell microenvironment, thus affecting the biomimetic design and regenerative efficiency. Mechanical properties are one of the essential aspects of microenvironment, which can be characterized by matrix stiffness, viscoelasticity, topography, and dynamic mechanical stimulation. With the recent advances in functional biomaterials, various engineered bio-inks have realized the possibility of engineering cell mechanical microenvironment In this review, we summarize the critical mechanical cues of cell microenvironments, review the engineered bio-inks while focusing on the selection principles for constructing cell mechanical microenvironments, and discuss the challenges facing this field and the possible solutions for them.
三维（3D）生物打印已成为一种很有前景的方法，可用于构建用于组织工程和再生医学的功能性仿生组织。在3D生物打印中，生物墨水对于构建细胞微环境至关重要，从而影响仿生设计和再生效率。力学性能是微环境的重要方面之一，可通过基质硬度、粘弹性、拓扑结构和动态力学刺激来表征。随着功能性生物材料的最新进展，各种工程化生物墨水已实现了构建细胞力学微环境的可能性。在本综述中，我们总结了细胞微环境的关键力学线索，回顾了工程化生物墨水，重点关注构建细胞力学微环境的选择原则，并讨论了该领域面临的挑战及其可能的解决方案。
