Ninsiima Herbert Izo, Eze Ejike Daniel, Ssekatawa Kenneth, Nalugo Halima, Asekenye Caroline, Onanyang David, Munanura Edson Ireeta, Ariong Moses, Matama Kevin, Zirintunda Gerald, Mbiydzenyuy Ngala Elvis, Ssempijja Fred, Afodun Adam Moyosore, Mujinya Regan, Usman Ibe Michael, Asiimwe Oscar Hilary, Tibyangye Julius, Kasozi Keneth Iceland
Department of Physiology, School of Medicine, Kabale University, Box 317, Kabale, Uganda.
Department of Veterinary Pharmacy, Clinics and Comparative Medicine, School of Veterinary Medicine and Animal Resources, Makerere University, Box 7062, Kampala, Uganda.
Heliyon. 2023 Feb 21;9(3):e13922. doi: 10.1016/j.heliyon.2023.e13922. eCollection 2023 Mar.
Information on the basic changes associated with green tea small molecules in acute inflammation is deficient. The purpose of the study was to characterize and establish the effects of green tea silver nanoparticles (AgNPs) following inflammation in male mice. In this study, green tea silver nitrate nanoparticles were characterized and the extract were made up to constitute high (100%), medium (10%), and low (1%) concentrations for administration. Acute inflammation was induced in groups I-V of the experimental rodents by injecting 0.5 ml/kg of fresh egg albumin on the subplantar surface of the right hind paw and animals were monitored for 36 h. Group I-III were administered 100%, 10%, 1% green tea nanoparticles extract while group IV was given diclofenac. Group V was the positive control while group VI was the negative control that received the vehicle. Paw edema was measured at a 2 h interval for 3 days, while the pain was assessed by measuring the locomotion activity using the voluntary wheel running and the anxiety-like behavior. Hypersensitivity was measured through the temperature sensation experiment and a non-linear regression analysis was done. Here, synthesized green tea AgNPs registered an absorbance band at 460 nm, phytochemicals due to presence of organic functional groups of O[bond, double bond]C[bond, double bond]O of oxycarbons, of C[bond, double bond]C of a conjugate alkene, C[bond, double bond]O of a stretching bond of a secondary alcohol. The silver green tea nanoparticles were spherical, covered by a slimy layer, capped and stable. Green tea AgNPs significantly decreased temperature hypersensitivity in male mice and this demonstrated their protective effects. Low concentrations of green tea nanoparticles inhibited edema thus mimicking effects of diclofenac, however, the percentage of inhibition was highest in medium and high silver-tea nanoparticles concentrations demonstration the importance of concentration in therapeutics. Anxiety was lowest in male mice treated with high concentrations of silver green tea nanoparticles, and this led to increased locomotory activity in mice. Green tea AgNPs have strong anti-inflammatory effects at high concentrations. Concentrations of green tea AgNPs modulated basic sensory and motor behaviors in male mice demonstrating their importance in complementary and integrative medical practice.
关于绿茶小分子在急性炎症中相关基本变化的信息不足。本研究的目的是表征并确定绿茶银纳米颗粒（AgNPs）对雄性小鼠炎症后的影响。在本研究中，对绿茶硝酸银纳米颗粒进行了表征，并将提取物配制成高（100%）、中（10%）、低（1%）浓度用于给药。通过在实验啮齿动物的右后爪足底皮下注射0.5 ml/kg新鲜蛋清诱导I - V组急性炎症，并对动物进行36小时监测。I - III组分别给予100%、10%、1%的绿茶纳米颗粒提取物，IV组给予双氯芬酸。V组为阳性对照，VI组为接受赋形剂的阴性对照。连续3天每隔2小时测量爪部水肿，同时通过使用自愿轮转跑步测量运动活动和焦虑样行为来评估疼痛。通过温度感觉实验测量超敏反应并进行非线性回归分析。在此，合成的绿茶AgNPs在460 nm处有吸收带，由于存在含氧碳的O[键，双键]C[键，双键]O有机官能团以及共轭烯烃的C[键，双键]C、仲醇拉伸键的C[键，双键]O而产生植物化学物质。绿茶银纳米颗粒呈球形，被一层黏液层覆盖，有帽且稳定。绿茶AgNPs显著降低了雄性小鼠的温度超敏反应，这证明了它们的保护作用。低浓度的绿茶纳米颗粒抑制水肿，从而模拟双氯芬酸的作用，然而，抑制百分比在中高浓度的银 - 茶纳米颗粒中最高，这表明浓度在治疗中的重要性。高浓度银绿茶纳米颗粒处理的雄性小鼠焦虑程度最低，这导致小鼠运动活动增加。绿茶AgNPs在高浓度时有很强的抗炎作用。绿茶AgNPs的浓度调节了雄性小鼠的基本感觉和运动行为，证明了它们在补充和综合医学实践中的重要性。
