Wang Min, Li Lili, Qian Jun, Wang Nannan, Bao Jun, Lu Jiangyue, Chen Faming, Li Yanfen, Zhang Yangheng, Yan Fuhua
Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210008, China.
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
iScience. 2023 Mar 7;26(4):106346. doi: 10.1016/j.isci.2023.106346. eCollection 2023 Apr 21.
Periodontitis may aggravate the development of nonalcoholic fatty liver disease (NAFLD); however, the precise mechanism is unknown. In this study, salivary microbiota collected from patients with periodontitis was transferred intragastrically to obese mice induced by high-fat diet. Microbiomics and metabolomics analysis were performed to assess the influence of periodontitis salivary microbiota on gut microbiome and liver metabolism. Periodontitis salivary microbiota altered gut microbiota composition and exacerbated intestinal barrier dysfunction in obese mice. Subsequently, the bacterial lipopolysaccharide transported to liver may activate the toll-like receptor 4 signaling and cause the release of pro-inflammatory factors. Moreover, the tryptophan-kynurenine-AhR signal axis was upregulated in liver, which may be related to aggravated hepatic steatosis and glucolipid metabolism dysregulation during NAFLD development. This study indicated that in the context of obesity, periodontitis salivary microbiota may aggravate the pathological progression of NAFLD, in which the tryptophan-AhR pathway may play a key role.
牙周炎可能会加重非酒精性脂肪性肝病（NAFLD）的发展；然而，确切机制尚不清楚。在本研究中，将从牙周炎患者收集的唾液微生物群经胃内转移至高脂饮食诱导的肥胖小鼠体内。进行了微生物组学和代谢组学分析，以评估牙周炎唾液微生物群对肠道微生物群和肝脏代谢的影响。牙周炎唾液微生物群改变了肥胖小鼠的肠道微生物群组成，并加剧了肠道屏障功能障碍。随后，转运至肝脏的细菌脂多糖可能激活Toll样受体4信号并导致促炎因子的释放。此外，肝脏中的色氨酸-犬尿氨酸-AhR信号轴上调，这可能与NAFLD发展过程中肝脂肪变性加重和糖脂代谢失调有关。本研究表明，在肥胖背景下，牙周炎唾液微生物群可能会加重NAFLD的病理进展，其中色氨酸-AhR途径可能起关键作用。
