Cavelius Philipp, Engelhart-Straub Selina, Biewald Alexander, Haack Martina, Awad Dania, Brueck Thomas, Mehlmer Norbert
TUM School of Natural Sciences, Technical University of Munich (TUM), 85748 Garching, Germany.
Microorganisms. 2023 Feb 22;11(3):553. doi: 10.3390/microorganisms11030553.
is a carotenogenic, oleogenic yeast that is able to grow in diverse environments. In this study, the proteomic and metabolic responses to copper stress in the two haplotypes IFO0559 and IFO0880 were assessed. 0.5 mM Cu(I) extended the lag phase of both strains significantly, while only a small effect was observed for Cu(II) treatment. Other carotenogenic yeasts such as are known to accumulate high amounts of carotenoids as a response to oxidative stress, posed by excess copper ion activity. However, no significant increase in carotenoid accumulation for both haplotypes of after 144 h of 0.5 mM Cu(I) or Cu(II) stress was observed. Yet, an increase in lipid production was detected, when exposed to Cu(II), additionally, proteins related to fatty acid biosynthesis were detected in increased amounts under stress conditions. Proteomic analysis revealed that besides the activation of the enzymatic oxidative stress response, excess copper affected iron-sulfur and zinc-containing proteins and caused proteomic adaptation indicative of copper ion accumulation in the vacuole, mitochondria, and Golgi apparatus.
是一种能合成类胡萝卜素、产油的酵母，能够在多种环境中生长。在本研究中，评估了两种单倍型IFO0559和IFO0880对铜胁迫的蛋白质组学和代谢反应。0.5 mM Cu(I)显著延长了两种菌株的延迟期，而Cu(II)处理仅观察到较小的影响。其他类胡萝卜素生成酵母，如已知会因过量铜离子活性引起的氧化应激而积累大量类胡萝卜素。然而，在0.5 mM Cu(I)或Cu(II)胁迫144小时后，两种单倍型的类胡萝卜素积累均未观察到显著增加。然而，当暴露于Cu(II)时，检测到脂质产量增加，此外，在应激条件下还检测到与脂肪酸生物合成相关的蛋白质数量增加。蛋白质组学分析表明，除了激活酶促氧化应激反应外，过量铜还影响含铁硫和含锌蛋白质，并导致蛋白质组适应，表明铜离子在液泡、线粒体和高尔基体中积累。
