呼吸道合胞病毒在细胞培养中的分离效果增强。
Enhanced isolation of respiratory syncytial virus in cell culture.
作者信息
Arens M Q, Swierkosz E M, Schmidt R R, Armstrong T, Rivetna K A
出版信息
J Clin Microbiol. 1986 Apr;23(4):800-2. doi: 10.1128/jcm.23.4.800-802.1986.
Abstract
During two winter seasons, we found that the combination of WI-38 or MRC-5 human lung fibroblasts plus primary rhesus monkey kidney (RhMK) and HEp-2 cell cultures yielded maximal isolation of respiratory syncytial virus. Cytopathic effects (CPE) developed earliest in RhMK cells and slowest in the human fibroblast lines. In RhMK cells, 50% of ultimately positive cultures showed CPE in 5 days, and 90% of positive cultures showed CPE within 7 days during both respiratory syncytial virus seasons.
摘要
在两个冬季期间，我们发现WI-38或MRC-5人肺成纤维细胞与原代恒河猴肾（RhMK）及HEp-2细胞培养物相结合，可实现呼吸道合胞病毒的最大程度分离。细胞病变效应（CPE）在RhMK细胞中出现最早，在人成纤维细胞系中出现最晚。在RhMK细胞中，在呼吸道合胞病毒流行的两个季节里，最终呈阳性的培养物中有50%在5天内出现CPE，90%的阳性培养物在7天内出现CPE。
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2
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