评估用于检测厌氧菌血症的血培养常规厌氧继代培养法。
Evaluation of a routine anaerobic subculture of blood cultures for detection of anaerobic bacteremia.
作者信息
Paisley J W, Rosenblatt J E, Hall M, Washington J A
出版信息
J Clin Microbiol. 1978 Dec;8(6):764-6. doi: 10.1128/jcm.8.6.764-766.1978.
Abstract
The value of a routine 48-h anaerobic subculture of blood cultures was assessed in our laboratory over a 4-month period. Excluding presumed contaminants, anaerobes represented 51 (6.9%) of the total number of 734 positive cultures. Sixteen isolates (all Bacteroides) from six patients were detected by the anaerobic subculture. All but one of these were also detected macroscopically. Excluding the one isolate, the routine anaerobic subculture hastened the identification of anaerobic organisms by only 1 day in two patients. We conclude that a routine anaerobic subculture is not indicated for the detection of anaerobic bacteremia.
摘要
在我们实验室，对为期4个月的血培养常规48小时厌氧亚培养的价值进行了评估。排除假定的污染物后，厌氧菌在734份阳性培养物总数中占51份（6.9%）。通过厌氧亚培养从6名患者中检测到16株分离菌（均为拟杆菌属）。除其中1株外，其余所有菌株在肉眼观察时也被检测到。排除这1株分离菌，常规厌氧亚培养仅使两名患者的厌氧菌鉴定提前了1天。我们得出结论，常规厌氧亚培养不适用于检测厌氧菌血症。
相似文献
1
Evaluation of a routine anaerobic subculture of blood cultures for detection of anaerobic bacteremia.评估用于检测厌氧菌血症的血培养常规厌氧继代培养法。
J Clin Microbiol. 1978 Dec;8(6):764-6. doi: 10.1128/jcm.8.6.764-766.1978.
2
Evaluation of routine subcultures of macroscopically negative blood cultures for detection of anaerobes.对肉眼观察为阴性的血培养物进行常规传代培养以检测厌氧菌的评估。
J Clin Microbiol. 1978 Oct;8(4):427-30. doi: 10.1128/jcm.8.4.427-430.1978.
3
Evaluation of the Minitek system for direct identification of anaerobic rods from positive blood cultures.用于直接从阳性血培养物中鉴定厌氧杆菌的Minitek系统评估。
J Clin Microbiol. 1982 Oct;16(4):673-5. doi: 10.1128/jcm.16.4.673-675.1982.
4
Initial detection of bacteremia by subculture of unvented tryptic soy broth blood culture bottles.通过未通气的胰蛋白酶大豆肉汤血培养瓶进行亚培养首次检测到菌血症。
Diagn Microbiol Infect Dis. 1984 Apr;2(2):107-11. doi: 10.1016/0732-8893(84)90005-1.
5
Optimal time for routine early subculture of blood cultures.血培养常规早期传代的最佳时间。
J Clin Microbiol. 1980 Sep;12(3):445-6. doi: 10.1128/jcm.12.3.445-446.1980.
6
Comparison of the BACTEC system with blind subculture for the detection of bacteremia.BACTEC系统与盲目传代培养法在检测菌血症方面的比较。
Appl Microbiol. 1974 Sep;28(3):435-8. doi: 10.1128/am.28.3.435-438.1974.
7
Anaerobic bacteremia.厌氧菌血症
Clin Infect Dis. 1996 Dec;23 Suppl 1:S97-101. doi: 10.1093/clinids/23.supplement_1.s97.
8
Numbers and types of anaerobic bacteria isolated from clinical specimens since 1960.自1960年以来从临床标本中分离出的厌氧细菌的数量和种类。
J Clin Microbiol. 1977 Jan;5(1):20-5. doi: 10.1128/jcm.5.1.20-25.1977.
9
Evaluation of blood culture procedures in a pediatric hospital.一家儿科医院血培养程序的评估
J Clin Microbiol. 1979 Jan;9(1):88-92. doi: 10.1128/jcm.9.1.88-92.1979.
10
Bacteroides fragilis bacteremia and infected aortic aneurysm presenting as fever of unknown origin: diagnostic delay without routine anaerobic blood cultures.脆弱拟杆菌血症和感染性主动脉瘤表现为不明原因发热：因未进行常规厌氧血培养导致诊断延迟
Clin Infect Dis. 1999 Nov;29(5):1309-11. doi: 10.1086/313429.
引用本文的文献
1
Update on detection of bacteremia and fungemia.菌血症和真菌血症检测的最新进展。
Clin Microbiol Rev. 1997 Jul;10(3):444-65. doi: 10.1128/CMR.10.3.444.
2
Advances in blood cultures.血培养的进展
Trans Am Clin Climatol Assoc. 1982;93:42-9.
3
Evaluation of the necessity for routine terminal subculturing of blood cultures negative by radiometric methods.评估通过放射性方法检测为阴性的血培养进行常规终末传代培养的必要性。
J Clin Microbiol. 1982 Jan;15(1):35-40. doi: 10.1128/jcm.15.1.35-40.1982.
4
Gas-liquid chromatography of positive blood cultures for rapid presumptive diagnosis of anaerobic bacteremia.用于厌氧血症快速初步诊断的阳性血培养物的气液色谱法。
J Clin Microbiol. 1982 Jun;15(6):1059-61. doi: 10.1128/jcm.15.6.1059-1061.1982.
5
Gas-liquid chromatography in routine processing of blood cultures for detecting anaerobic bacteraemia.用于检测厌氧菌血症的血培养常规处理中的气液色谱法。
J Clin Pathol. 1981 Feb;34(2):189-93. doi: 10.1136/jcp.34.2.189.
6
Optimal time for routine early subculture of blood cultures.血培养常规早期传代的最佳时间。
J Clin Microbiol. 1980 Sep;12(3):445-6. doi: 10.1128/jcm.12.3.445-446.1980.
7
Evaluation of the necessity for routine terminal subcultures of previously negative blood cultures.评估既往血培养阴性时进行常规终末传代培养的必要性。
J Clin Microbiol. 1980 Oct;12(4):576-8. doi: 10.1128/jcm.12.4.576-578.1980.
8
Clinical laboratory comparison of a slide blood culture system with a conventional broth system.玻片血培养系统与传统肉汤系统的临床实验室比较
J Clin Microbiol. 1982 Sep;16(3):525-30. doi: 10.1128/jcm.16.3.525-530.1982.
9
Critical assessment of blood culture techniques: analysis of recovery of obligate and facultative anaerobes, strict aerobic bacteria, and fungi in aerobic and anaerobic blood culture bottles.血培养技术的批判性评估：需氧和厌氧血培养瓶中 obligate 和兼性厌氧菌、严格需氧菌及真菌的回收率分析
J Clin Microbiol. 1992 Jun;30(6):1462-8. doi: 10.1128/jcm.30.6.1462-1468.1992.
10
Evaluation of a biphasic medium for blood cultures.用于血培养的双相培养基的评估
J Clin Microbiol. 1979 Nov;10(5):673-6. doi: 10.1128/jcm.10.5.673-676.1979.
本文引用的文献
1
Growth patterns of a wide spectrum of organisms encountered in clinical blood cultures using both hypertonic and isotonic media.使用高渗和等渗培养基的临床血培养中遇到的多种生物体的生长模式。
Am J Clin Pathol. 1976 May;65(5):706-10. doi: 10.1093/ajcp/65.5.706.
2
Anaerobic bacteremia.厌氧菌血症
Mayo Clin Proc. 1972 Sep;47(9):639-46.
3
Comparison of two liquid blood culture media containing sodium polyanethole sulfonate: tryptic soy and Columbia.两种含聚茴香脑磺酸钠的液体血液培养基的比较：胰蛋白酶大豆培养基和哥伦比亚培养基。
Appl Microbiol. 1974 Apr;27(4):699-702. doi: 10.1128/am.27.4.699-702.1974.
4
Detection of bacteremia with liquid media containing sodium polyanetholsulfonate.使用含聚茴香脑磺酸钠的液体培养基检测菌血症。
Appl Microbiol. 1974 Jan;27(1):187-91. doi: 10.1128/am.27.1.187-191.1974.
5
Comparison of macroscopic examination, routine gram stains, and routine subcultures in the initial detection of positive blood cultures.在血培养阳性的初始检测中，宏观检查、常规革兰氏染色和常规传代培养的比较。
Appl Microbiol. 1974 Mar;27(3):537-9. doi: 10.1128/am.27.3.537-539.1974.
6
Effects of atmosphere of incubation and of routine subcultures on detection of bacteremia in vacuum blood culture bottles.培养环境及常规传代培养对真空血培养瓶中菌血症检测的影响。
J Clin Microbiol. 1975 Oct;2(4):296-9. doi: 10.1128/jcm.2.4.296-299.1975.
7
Rapid detection of bacteremia by an early subculture technic.采用早期传代培养技术快速检测菌血症。
Am J Clin Pathol. 1975 Nov;64(5):694-9. doi: 10.1093/ajcp/64.5.694.
8
Critical analysis of hypertonic medium and agitation in detection of bacteremia.高渗培养基及振荡在菌血症检测中的批判性分析
J Clin Microbiol. 1976 Sep;4(3):216-24. doi: 10.1128/jcm.4.3.216-224.1976.
9
Comparative evaluation of three different commercial blood culture media for recovery of anaerobic organisms.三种不同商业血培养培养基对厌氧菌分离效果的比较评估
J Clin Microbiol. 1977 May;5(5):505-9. doi: 10.1128/jcm.5.5.505-509.1977.
10
Isolation and identification of anaerobic bacteria.厌氧菌的分离与鉴定
Hum Pathol. 1976 Mar;7(2):177-86. doi: 10.1016/s0046-8177(76)80021-4.
Zotero 插件