Royston D, Fleming J S, Desai J B, Westaby S, Taylor K M
J Thorac Cardiovasc Surg. 1986 May;91(5):759-66.
We investigated the degree and time course of neutrophil sequestration into human lungs during cardiac operations. At the same time, measurement of the concentration of peroxidation products in the plasma was used as an index of oxidant free radical activity. The study was performed in two groups of patients. Group A (n = 11) had studies extending over the entire operative period and showed a highly significant sequestration of neutrophils into the lung, together with a highly significant (p less than 0.001) rise in peroxidation products from 2.8 +/- 0.12 nmol/ml(mean +/- standard error of the mean)before bypass to a peak of 5.05 +/- 0.13 nmol/ml at the end of bypass. As these changes occurred only during the time after release of the aortic cross-clamp, we investigated this period in more detail in a second group of patients (Group B, n = 7). Results from this group showed that significant release of peroxidation products occurred at the same time as pulmonary neutrophil sequestration. This study has produced evidence of increased oxidant activity in the lung associated with cardiac operations. Nevertheless, it is not known whether the neutrophils sequestered into the lung alone induced the increased activity. Similarly, whether neutrophil-derived oxidant species are the sole cause of lung tissue injury remains unproved.
我们研究了心脏手术期间中性粒细胞在人肺内滞留的程度和时间进程。同时，测定血浆中过氧化产物的浓度作为氧化自由基活性的指标。该研究在两组患者中进行。A组（n = 11）的研究涵盖整个手术期，结果显示中性粒细胞在肺内有高度显著的滞留，同时过氧化产物也有高度显著（p < 0.001）的升高，从体外循环前的2.8±0.12 nmol/ml（均值±均值标准误）升至体外循环结束时的峰值5.05±0.13 nmol/ml。由于这些变化仅发生在主动脉阻断钳松开后的时间段，我们在第二组患者（B组，n = 7）中更详细地研究了这一时期。该组结果显示，过氧化产物的显著释放与肺内中性粒细胞的滞留同时发生。本研究已证明与心脏手术相关的肺内氧化活性增加。然而，尚不清楚单独滞留在肺内的中性粒细胞是否会诱导活性增加。同样，中性粒细胞衍生的氧化物质是否是肺组织损伤的唯一原因仍未得到证实。
