Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, China.
Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, China.
Genome Biol. 2023 Apr 6;24(1):67. doi: 10.1186/s13059-023-02918-9.
N-methyladenosine (mA) modification has been shown to regulate RNA metabolism. Here, we investigate mA dynamics during maternal-to-zygotic transition (MZT) in mice through multi-omic analysis. Our results show that mA can be maternally inherited or de novo gained after fertilization. Interestingly, mA modification on maternal mRNAs not only correlates with mRNA degradation, but also maintains the stability of a small group of mRNAs thereby promoting their translation after fertilization. We identify Ythdc1 and Ythdf2 as key mA readers for mouse preimplantation development. Our study reveals a key role of mA mediated RNA metabolism during MZT in mammals.
N6-甲基腺苷（m6A）修饰被证明可以调节 RNA 代谢。在这里，我们通过多组学分析研究了小鼠母源到合子过渡（MZT）期间的 m6A 动态。我们的结果表明，m6A 可以在受精后从母本遗传或从头获得。有趣的是，母源 mRNA 上的 m6A 修饰不仅与 mRNA 降解相关，而且还能维持一小部分 mRNA 的稳定性，从而促进它们在受精后的翻译。我们确定 Ythdc1 和 Ythdf2 是小鼠胚胎植入前发育的关键 m6A 读取器。我们的研究揭示了 m6A 介导的 RNA 代谢在哺乳动物 MZT 中的关键作用。
