Huang Ssu-Meng, Liu Shih-Ming, Tseng Hua-Yi, Chen Wen-Cheng
Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, Feng Chia University, Taichung 407, Taiwan.
Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Polymers (Basel). 2023 Mar 31;15(7):1738. doi: 10.3390/polym15071738.
A series of electrospun polyvinyl alcohol (PVA) fiber membranes were crosslinked with citric acid (CA) at concentrations of 10, 20, and 30 wt.% (designated as CA10, CA20, and CA30). The effects of CA on the chemical structure, mechanical strength, swelling resistance, and cytotoxicity of the crosslinked PVA fibrous membranes were investigated. Infrared spectroscopy indicated the enhanced esterification of carboxyl and hydroxyl groups between CA and PVA. The modulus and strength of the electrospun PVA membrane increased due to the crosslinking between CA and PVA. The crosslinking of the PVA fiber matrix with CA increased the PVA binding point, thereby increasing the swelling resistance and modulus; however, the concentration of CA used was limited. Results showed that the water absorption of the PVA membranes decreased from 6.58 ± 0.04 g/g for CA10 to 3.56 ± 3.33 g/g for CA20 and 2.85 ± 0.40 g/g for CA30 with increasing CA. The water absorption remained unchanged after the membrane was soaked for a period of time, so no significant difference was found in the water absorption capacity of the same group after immersion from 1 h to 3 d. The tensile strength increased from 20.52 MPa of CA10 to 22.09 MPa of CA20. With an increased amount of CA used for crosslinking, the tensile strength and modulus of CA30 decreased to 11.48 and 13.94 MPa, respectively. Our study also showed that CA was not toxic to L929 cell viability when used for fiber crosslinking at less than 20 wt.% PVA, meaning it may be a good candidate as a support layer for guided tissue engineering.
一系列静电纺丝聚乙烯醇（PVA）纤维膜与浓度为10%、20%和30%（重量）的柠檬酸（CA）进行交联（分别命名为CA10、CA20和CA30）。研究了CA对交联PVA纤维膜的化学结构、机械强度、抗溶胀性和细胞毒性的影响。红外光谱表明CA与PVA之间羧基和羟基的酯化作用增强。由于CA与PVA之间的交联，静电纺丝PVA膜的模量和强度增加。PVA纤维基质与CA的交联增加了PVA的结合点，从而提高了抗溶胀性和模量；然而，所用CA的浓度是有限的。结果表明，随着CA含量的增加，PVA膜的吸水率从CA10的6.58±0.04 g/g降至CA20的3.56±3.33 g/g和CA30的2.85±0.40 g/g。膜浸泡一段时间后吸水率保持不变，因此同一组膜在浸泡1小时至3天期间的吸水能力没有显著差异。拉伸强度从CA10的20.52 MPa增加到CA20的22.09 MPa。随着用于交联的CA量增加，CA30的拉伸强度和模量分别降至11.48和13.94 MPa。我们的研究还表明，当用于PVA含量低于20%（重量）的纤维交联时，CA对L929细胞活力无毒，这意味着它可能是引导组织工程支撑层的良好候选材料。
