肠道螺旋体的分离与鉴定
Isolation and characterisation of intestinal spirochaetes.
作者信息
Tompkins D S, Foulkes S J, Godwin P G, West A P
出版信息
J Clin Pathol. 1986 May;39(5):535-41. doi: 10.1136/jcp.39.5.535.
Abstract
Faeces or rectal swabs from 1527 subjects were examined for the presence of intestinal spirochaetes by anaerobic culture on blood agar incorporating spectinomycin (400 mg/l). Twenty three specimens (1.5%) were positive, and only one of these came from a patient with diarrhoea. All positive specimens came from either Asians or known homosexuals. Comparative tests showed a close phenotypic similarity between the human isolates and non-pathogenic porcine intestinal spirochaetes. These organisms differ from Brachyspira aalborgi, a spirochaete isolated from subjects with histologically confirmed intestinal spirochaetosis.
摘要
对1527名受试者的粪便或直肠拭子进行检测，通过在含有壮观霉素（400mg/L）的血琼脂上进行厌氧培养，检查是否存在肠道螺旋体。23份标本（1.5%）呈阳性，其中只有一份来自腹泻患者。所有阳性标本均来自亚洲人或已知的同性恋者。对比试验表明，人类分离株与非致病性猪肠道螺旋体在表型上非常相似。这些微生物与从组织学确诊的肠道螺旋体病患者中分离出的螺旋体——奥尔堡短螺旋体不同。
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