Stenlund Patrik, Enstedt Linnea, Gilljam Karin Margaretha, Standoft Simon, Ahlinder Astrid, Lundin Johnson Maria, Lund Henrik, Millqvist Fureby Anna, Berglin Mattias
Department of Methodology, Textile and Medical Technology, RISE Research Institutes of Sweden AB, Arvid Wallgrens backe 20, SE-413 46 Gothenburg, Sweden.
Department of Chemical Process and Pharmaceutical Development, RISE Research Institutes of Sweden AB, Drottning Kristinas väg 61B, SE-114 28 Stockholm, Sweden.
Polymers (Basel). 2023 Jun 9;15(12):2627. doi: 10.3390/polym15122627.
Current standard wound care involves dressings that provide moisture and protection; however, dressings providing active healing are still scarce and expensive. We aimed to develop an ecologically sustainable 3D printed bioactive hydrogel-based topical wound dressing targeting healing of hard-to-heal wounds, such as chronic or burn wounds, which are low on exudate. To this end, we developed a formulation composed of renewable marine components; purified extract from unfertilized salmon roe (heat-treated X, HTX), alginate from brown seaweed, and nanocellulose from tunicates. HTX is believed to facilitate the wound healing process. The components were successfully formulated into a 3D printable ink that was used to create a hydrogel lattice structure. The 3D printed hydrogel showed a HTX release profile enhancing pro-collagen I alpha 1 production in cell culture with potential of promoting wound closure rates. The dressing has recently been tested on burn wounds in Göttingen minipigs and shows accelerated wound closure and reduced inflammation. This paper describes the dressings development, mechanical properties, bioactivity, and safety.
当前的标准伤口护理涉及提供保湿和保护的敷料；然而，具有促进愈合活性的敷料仍然稀缺且昂贵。我们旨在开发一种生态可持续的、基于3D打印生物活性水凝胶的局部伤口敷料，用于治疗难愈合伤口，如慢性伤口或烧伤伤口，这些伤口渗出液较少。为此，我们开发了一种由可再生海洋成分组成的配方；未受精鲑鱼卵的纯化提取物（热处理X，HTX）、褐藻中的藻酸盐和被囊动物中的纳米纤维素。据信HTX有助于伤口愈合过程。这些成分被成功配制成一种3D可打印墨水，用于创建水凝胶晶格结构。3D打印水凝胶显示出HTX释放曲线，可提高细胞培养中I型前胶原α1的产生，具有提高伤口闭合率的潜力。该敷料最近已在哥廷根小型猪的烧伤伤口上进行测试，显示出加速伤口闭合和减轻炎症的效果。本文描述了该敷料的开发、力学性能、生物活性和安全性。
