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两个对比品种发育胚中糖积累差异的转录组分析

Transcriptome analysis of differential sugar accumulation in the developing embryo of contrasting two cultivars.

作者信息

Huang Ruimin, Peng Fei, Wang Dongsheng, Cao Fei, Guo Chunlei, Yu Liyang, Zhang Jingzheng, Yang Yuedong

机构信息

Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, China.

Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, China.

出版信息

Front Plant Sci. 2023 Jun 19;14:1206585. doi: 10.3389/fpls.2023.1206585. eCollection 2023.

DOI:10.3389/fpls.2023.1206585
PMID:37404530
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10315843/
Abstract

Chinese chestnut () is an important nut tree species, and its embryo is rich in sugar. We combined metabolomic and transcriptomic data to analyze metabolites and genes related to sugar in two Chinese chestnut cultivars at 60, 70, 80, 90 and 100 days after flowering (DAF). The soluble sugar content of high-sugar cultivar at maturity is 1.5 times that of low-sugar cultivar. Thirty sugar metabolites were identified in embryo, with the most dominant being sucrose. Analysis of the gene expression patterns revealed that the high-sugar cultivar promoted the conversion of starch to sucrose by up-regulating genes related to starch degradation and sucrose synthesis at 90-100 DAF. It also strongly increased the enzyme activity of SUS-synthetic, which may promote sucrose synthesis. Gene co-expression network analysis showed that ABA and peroxide were related to starch decomposition during Chinese chestnut ripening. Our study analyzed the composition and molecular synthesis mechanism of sugar in Chinese chestnut embryos, and provided a new insight into the regulation pattern of high sugar accumulation in Chinese chestnut nuts.

摘要

板栗（）是一种重要的坚果树种，其胚富含糖类。我们结合代谢组学和转录组学数据，分析了两个板栗品种在开花后60、70、80、90和100天（DAF）时与糖类相关的代谢物和基因。成熟时高糖品种的可溶性糖含量是低糖品种的1.5倍。在胚中鉴定出30种糖代谢物，其中最主要的是蔗糖。对基因表达模式的分析表明，高糖品种在开花后90 - 100天通过上调与淀粉降解和蔗糖合成相关的基因来促进淀粉向蔗糖的转化。它还显著提高了SUS合成酶的活性，这可能促进蔗糖合成。基因共表达网络分析表明，脱落酸和过氧化物与板栗成熟过程中的淀粉分解有关。我们的研究分析了板栗胚中糖类的组成和分子合成机制，为板栗坚果中高糖积累的调控模式提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/784e35a419ad/fpls-14-1206585-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/c9bd22f461c7/fpls-14-1206585-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/23ac93cf1a7a/fpls-14-1206585-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/ca0f71fa420a/fpls-14-1206585-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/9956e92f6db7/fpls-14-1206585-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/94f6fee7b7eb/fpls-14-1206585-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/d63cf8acf620/fpls-14-1206585-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/d38cd30f3766/fpls-14-1206585-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/77649363e586/fpls-14-1206585-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/420c8da78c69/fpls-14-1206585-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/784e35a419ad/fpls-14-1206585-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/c9bd22f461c7/fpls-14-1206585-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/23ac93cf1a7a/fpls-14-1206585-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/ca0f71fa420a/fpls-14-1206585-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/9956e92f6db7/fpls-14-1206585-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/94f6fee7b7eb/fpls-14-1206585-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/d63cf8acf620/fpls-14-1206585-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/d38cd30f3766/fpls-14-1206585-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/77649363e586/fpls-14-1206585-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/420c8da78c69/fpls-14-1206585-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9249/10315843/784e35a419ad/fpls-14-1206585-g010.jpg

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