• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

外加电场诱导细胞膜电位变化的光学成像。

Optical imaging of cell membrane potential changes induced by applied electric fields.

作者信息

Gross D, Loew L M, Webb W W

出版信息

Biophys J. 1986 Aug;50(2):339-48. doi: 10.1016/S0006-3495(86)83467-1.

DOI:10.1016/S0006-3495(86)83467-1
PMID:3741986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1329750/
Abstract

We report the first imaging of the spatial distributions of transmembrane potential changes induced in nonexcitable cells by applied external electric fields. These changes are indicated by the fluorescence intensity of a charge-shift potentiometric dye incorporated in the cell plasma membrane and measured by digital intensified video microscopy.

摘要

我们报告了首次对由外部施加电场在非可兴奋细胞中诱导产生的跨膜电位变化的空间分布进行成像。这些变化通过掺入细胞质膜中的电荷转移电位染料的荧光强度来指示，并通过数字增强视频显微镜进行测量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/fee3c29182f4/biophysj00176-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/332d89e06374/biophysj00176-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/0a3233daeeed/biophysj00176-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/1ddc6cb0191c/biophysj00176-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/fee3c29182f4/biophysj00176-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/332d89e06374/biophysj00176-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/0a3233daeeed/biophysj00176-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/1ddc6cb0191c/biophysj00176-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4f9/1329750/fee3c29182f4/biophysj00176-0136-a.jpg

相似文献

1
Optical imaging of cell membrane potential changes induced by applied electric fields.外加电场诱导细胞膜电位变化的光学成像。
Biophys J. 1986 Aug;50(2):339-48. doi: 10.1016/S0006-3495(86)83467-1.
2
Membrane electric properties by combined patch clamp and fluorescence ratio imaging in single neurons.在单个神经元中通过膜片钳和荧光比率成像相结合的方法研究膜电特性。
Biophys J. 1998 Jan;74(1):48-53. doi: 10.1016/S0006-3495(98)77765-3.
3
Membrane potential induced by external electric field pulses can be followed with a potentiometric dye.外部电场脉冲诱导的膜电位可以用电位染料进行监测。
Biophys J. 1987 May;51(5):833-7. doi: 10.1016/S0006-3495(87)83410-0.
4
Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential.通过跨膜电位亚微秒成像分析电穿孔细胞的膜电导。
Biophys J. 1991 Jan;59(1):209-20. doi: 10.1016/S0006-3495(91)82212-3.
5
Measuring the induced membrane voltage with Di-8-ANEPPS.用Di-8-ANEPPS测量诱导膜电压。
J Vis Exp. 2009 Nov 19(33):1659. doi: 10.3791/1659.
6
Osmotically evoked shrinking of guard-cell protoplasts causes vesicular retrieval of plasma membrane into the cytoplasm.渗透诱导保卫细胞原生质体收缩，导致质膜以囊泡形式内吞进入细胞质。
Planta. 2000 Feb;210(3):423-31. doi: 10.1007/PL00008151.
7
Transmembrane potential measurements on plant cells using the voltage-sensitive dye ANNINE-6.利用膜电压敏感性染料 ANNINE-6 对植物细胞进行跨膜电位测量。
Protoplasma. 2010 Nov;247(1-2):3-12. doi: 10.1007/s00709-010-0131-y. Epub 2010 Mar 23.
8
Dual-wavelength ratiometric fluorescence measurements of membrane potential.膜电位的双波长比率荧光测量
Biochemistry. 1989 May 30;28(11):4536-9. doi: 10.1021/bi00437a003.
9
Dielectrophoretic spectra of single cells determined by feedback-controlled levitation.通过反馈控制悬浮测定的单细胞介电泳谱。
Biophys J. 1990 Feb;57(2):173-82. doi: 10.1016/S0006-3495(90)82520-0.
10
Fluorescence emission spectral shift measurements of membrane potential in single cells.单细胞中膜电位的荧光发射光谱位移测量。
Biophys J. 2001 Aug;81(2):1163-70. doi: 10.1016/S0006-3495(01)75773-6.

引用本文的文献

1
Excitation and electroporation in genetically engineered excitable S-HEK cells exposed to electric pulses of different durations.暴露于不同持续时间电脉冲下的基因工程可兴奋S-HEK细胞中的激发和电穿孔。
Sci Rep. 2025 Jul 2;15(1):23451. doi: 10.1038/s41598-025-06989-5.
2
Managing surface energy dynamics for enhanced axonal growth: An overview of present and future challenges.调控表面能动力学以促进轴突生长：当前与未来挑战概述
Biophys Rev (Melville). 2025 Apr 30;6(2):021301. doi: 10.1063/5.0237085. eCollection 2025 Jun.
3
Seeing the Spikes: The Future of Targetable Synthetic Voltage Sensors.

本文引用的文献

1
Enhanced spinal cord regeneration in lamprey by applied electric fields.施加电场促进七鳃鳗脊髓再生。
Science. 1981 Aug 7;213(4508):611-7. doi: 10.1126/science.7256258.
2
Improvements in optical methods for measuring rapid changes in membrane potential.测量膜电位快速变化的光学方法的改进。
J Membr Biol. 1981 Feb 15;58(2):123-37. doi: 10.1007/BF01870975.
3
Improved fluorescent probes for the measurement of rapid changes in membrane potential.用于测量膜电位快速变化的改进型荧光探针。
洞察尖峰信号：可靶向合成电压传感器的未来。
ACS Chem Neurosci. 2025 Mar 5;16(5):761-771. doi: 10.1021/acschemneuro.4c00849. Epub 2025 Feb 13.
4
Calculating transmembrane voltage on the electric pulse-affected cancerous cell membrane: using molecular dynamics and finite element simulations.计算受电脉冲影响的癌细胞膜的跨膜电压：使用分子动力学和有限元模拟。
J Mol Model. 2024 Jun 21;30(7):221. doi: 10.1007/s00894-024-06012-0.
5
Membrane polarization at the excitation threshold induced by external electric fields in cardiomyocytes of rats at different developmental stages.不同发育阶段大鼠心肌细胞在外部电场作用下达到兴奋阈时的膜极化。
Med Biol Eng Comput. 2023 Oct;61(10):2637-2647. doi: 10.1007/s11517-023-02868-1. Epub 2023 Jul 5.
6
An evolutionarily conserved pacemaker role for HCN ion channels in smooth muscle.离子通道 HCN 在平滑肌中作为进化上保守的起搏器的作用。
J Physiol. 2023 Apr;601(7):1225-1246. doi: 10.1113/JP283701. Epub 2023 Mar 17.
7
Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values.细胞膜电位的荧光成像：从相对变化到绝对值。
Int J Mol Sci. 2023 Jan 26;24(3):2435. doi: 10.3390/ijms24032435.
8
Inducing Vertebrate Limb Regeneration: A Review of Past Advances and Future Outlook.诱导脊椎动物肢体再生：回顾过去的进展和未来展望。
Cold Spring Harb Perspect Biol. 2022 May 17;14(4):a040782. doi: 10.1101/cshperspect.a040782.
9
Visualization of Dynamic Sub-microsecond Changes in Membrane Potential.可视化膜电位的动态亚微秒变化。
Biophys J. 2019 Jan 8;116(1):120-126. doi: 10.1016/j.bpj.2018.11.3129. Epub 2018 Dec 1.
10
Bringing new dimensions to drug discovery screening: impact of cellular stimulation technologies.为药物发现筛选带来新维度：细胞刺激技术的影响
Drug Discov Today. 2017 Jul;22(7):1045-1055. doi: 10.1016/j.drudis.2017.01.015. Epub 2017 Feb 4.
Biophys J. 1982 Sep;39(3):301-8. doi: 10.1016/S0006-3495(82)84520-7.
4
Design and characterization of electrochromic membrane probes.电致变色膜探针的设计与表征
J Biochem Biophys Methods. 1982 Aug;6(3):243-60. doi: 10.1016/0165-022x(82)90047-1.
5
Electric pulse-induced fusion of 3T3 cells in monolayer culture.单层培养的3T3细胞的电脉冲诱导融合。
Science. 1982 Apr 30;216(4545):537-8. doi: 10.1126/science.7071601.
6
Electric field-induced cell-to-cell fusion.电场诱导的细胞间融合。
J Membr Biol. 1982;67(3):165-82. doi: 10.1007/BF01868659.
7
In situ electrophoresis of membrane components.膜成分的原位电泳
Annu Rev Biophys Bioeng. 1981;10:245-76. doi: 10.1146/annurev.bb.10.060181.001333.
8
The role of electro-osmosis in the electric-field-induced movement of charged macromolecules on the surfaces of cells.电渗在电场诱导带电大分子在细胞表面移动中的作用。
Biophys J. 1981 Apr;34(1):85-93. doi: 10.1016/S0006-3495(81)84838-2.
9
Fluorescence monitoring of electrical responses from small neurons and their processes.对小神经元及其突起的电反应进行荧光监测。
Biophys J. 1983 May;42(2):195-8. doi: 10.1016/S0006-3495(83)84386-0.
10
Calcium-dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields.暴露于强电场后牛肾上腺髓质细胞儿茶酚胺释放的钙依赖性
J Membr Biol. 1982;68(2):107-40. doi: 10.1007/BF01872259.