外加电场诱导细胞膜电位变化的光学成像。
Optical imaging of cell membrane potential changes induced by applied electric fields.
作者信息
Gross D, Loew L M, Webb W W
出版信息
Biophys J. 1986 Aug;50(2):339-48. doi: 10.1016/S0006-3495(86)83467-1.
Abstract
We report the first imaging of the spatial distributions of transmembrane potential changes induced in nonexcitable cells by applied external electric fields. These changes are indicated by the fluorescence intensity of a charge-shift potentiometric dye incorporated in the cell plasma membrane and measured by digital intensified video microscopy.
摘要
我们报告了首次对由外部施加电场在非可兴奋细胞中诱导产生的跨膜电位变化的空间分布进行成像。这些变化通过掺入细胞质膜中的电荷转移电位染料的荧光强度来指示，并通过数字增强视频显微镜进行测量。
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