Alali Arkan, Hosseini-Abari Afrouzossadat, Bahrami Abbas, Yazdan Mehr Maryam
Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan 81746-73441, Iran.
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Materials (Basel). 2023 Jun 28;16(13):4670. doi: 10.3390/ma16134670.
Modification of paint with nanoparticles (NPs) provides self-cleaning, water/dirt-repellent, and other properties. Therefore, the aim of the present study was to biosynthesize silver (Ag) and copper oxide (CuO) NPs and to prepare NP-modified paint. To this end, AgNPs and CuONPs were biosynthesized using spores and commercial and crude dipicolinic acid (DPA) extracted from the spore of this bacterium. The synthesized NPs were characterized using electron microscopy, Fourier-transform infrared (FTIR), X-ray diffraction analysis (XRD), and energy-dispersive X-ray spectroscopy (EDS) methods. A minimum inhibitory concentration (MIC) assay of NPs against ATCC8739 and ATCC6538 was carried out. The antibacterial effects of prepared NP-paint complexes were assessed using an optical density (OD) comparison before and after adding metal sheets coated with NP-paint complexes into the nutrient broth medium. Four different types of NPs were synthesized in this research: AgNPs synthesized by spore (A), AgNPs synthesized by commercial DPA (B), AgNPs synthesized by crude DPA (C), and CuONPs synthesized by spore (D). SEM analysis confirmed the spherical shape of NPs. According to the results, NPs A, B, and D showed higher antibacterial activity against compared to . Furthermore, the analysis of the antibacterial effects of NP-paint complexes suggested that paint-NPs A, B, and C displayed higher activity on compared to . Moreover, the antibacterial effect of paint-NP D was significantly lower than other NPs. According to this robust antibacterial effect on pathogenic bacteria, it seems that these NP-paint complexes could be useful in public places such as hospitals, airports, dormitories, schools, and office buildings, where the rate of transmission of infection is high.
用纳米颗粒（NPs）对涂料进行改性可提供自清洁、防水/防污等性能。因此，本研究的目的是生物合成银（Ag）和氧化铜（CuO）纳米颗粒，并制备纳米颗粒改性涂料。为此，利用芽孢以及从该细菌芽孢中提取的商业和粗制二吡啶甲酸（DPA）生物合成了AgNPs和CuONPs。使用电子显微镜、傅里叶变换红外（FTIR）、X射线衍射分析（XRD）和能量色散X射线光谱（EDS）方法对合成的纳米颗粒进行了表征。对纳米颗粒针对ATCC8739和ATCC6538进行了最低抑菌浓度（MIC）测定。通过将涂有纳米颗粒-涂料复合物的金属片添加到营养肉汤培养基前后的光密度（OD）比较，评估了制备的纳米颗粒-涂料复合物的抗菌效果。本研究合成了四种不同类型的纳米颗粒：由芽孢合成的AgNPs（A）、由商业DPA合成的AgNPs（B）、由粗制DPA合成的AgNPs（C）以及由芽孢合成的CuONPs（D）。扫描电子显微镜分析证实了纳米颗粒的球形形状。结果表明，与……相比，纳米颗粒A、B和D对……显示出更高的抗菌活性。此外，对纳米颗粒-涂料复合物抗菌效果的分析表明，与……相比，涂料-纳米颗粒A、B和C对……表现出更高的活性。此外，涂料-纳米颗粒D的抗菌效果明显低于其他纳米颗粒。鉴于对病原菌具有这种强大的抗菌作用，这些纳米颗粒-涂料复合物似乎可用于医院、机场、宿舍、学校和办公楼等公共场所，这些地方感染传播率较高。
