Stambolov Ivan, Shkondrov Aleksandar, Kunert Olaf, Bucar Franz, Kondeva-Burdina Magdalena, Krasteva Ilina
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav St., 1000 Sofia, Bulgaria.
Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 1, A-8010 Graz, Austria.
Metabolites. 2023 Jul 19;13(7):857. doi: 10.3390/metabo13070857.
(Fabaceae) is used in the traditional medicine of many countries against hepatic and cardiac disorders. The plant contains mainly flavonoids and saponins. From a defatted methanol extract from its overground parts, a new triterpenoid saponin, 3--[-L-rhamnopyranosyl-(1→2)]--D-xylopyranosyl]-24---L-arabinopyranosyl-3,6,16,24(),25-pentahydroxy-20-cycloartane, together with the rare saponin astrachrysoside A, were isolated using various chromatography methods. The compounds were identified via extensive high resolution electrospray ionisation mass spectrometry (HRESIMS) and NMR analyses. Both saponins were examined for their possible antioxidant and neuroprotective activity in three different in vitro models. Rat brain synaptosomes, mitochondria, and microsomes were isolated via centrifugation using Percoll gradient. They were treated with the compounds in three different concentrations alone, and in combination with 6-hydroxydopamine or -butyl hydroperoxide as toxic agents. It was found that the compounds had statistically significant dose-dependent in vitro protective activity on the sub-cellular fractions. The compounds exhibited a weak inhibitory effect on the enzyme activity of human recombinant monoamine oxidase type B (hMAO-B), compared to selegiline.
豆科植物在许多国家的传统医学中用于治疗肝脏和心脏疾病。该植物主要含有黄酮类化合物和皂苷。从其地上部分的脱脂甲醇提取物中，使用各种色谱方法分离出一种新的三萜皂苷，3-[-L-鼠李糖基-(1→2)]--D-木糖基]-24---L-阿拉伯糖基-3,6,16,24(),25-五羟基-20-环阿尔廷烷，以及罕见的皂苷紫菀皂苷A。通过广泛的高分辨率电喷雾电离质谱(HRESIMS)和核磁共振分析对这些化合物进行了鉴定。在三种不同的体外模型中检测了这两种皂苷的潜在抗氧化和神经保护活性。通过使用Percoll梯度离心法分离大鼠脑突触体、线粒体和微粒体。它们分别用三种不同浓度的化合物处理，并与6-羟基多巴胺或叔丁基过氧化氢作为有毒剂联合处理。结果发现，这些化合物对亚细胞组分具有统计学上显著的剂量依赖性体外保护活性。与司来吉兰相比，这些化合物对人重组单胺氧化酶B型(hMAO-B)的酶活性表现出较弱的抑制作用。
